Effects Of Simvastatin On TGF-β1 / Smad Signali Ng Pathway In The Process Of The Differentiation Of Conclusion

ObjectiveTo investgate whether simvastatin(SIM) promotes bone marrow mesenchymal stem cells(BMSCs) to differentiate to osteablasts by regulating TGF-β1/Smad signaling pathways.MethodsRat BMSCs were recovered and cultured, then were induced to differentiate into osteoblasts.In the experimental group given different concentrations of SIM(10-8 mol/L、10-7 mol/L、10-6 mol/L),the control group was not given drug intervention.Afetr 7 days with joining ostrogenic solution using alkaline phosphatase staining observed the expression level of bone formation,using Alizarin Red staining calcified horizontal,using Immunocytochemistry and Western blot detected the expression of protein levels of TGF-β1,Smad2 and Smad3.ResultsTreatment with SIM at concentrations of 10-8mol/L to10-6 mol/L for 7d obviously increased the activity of ALP, and SIM at concentration of 10-7 mol/L produced the maximum effect. Exposure of the cells to SIM at concentration of 10-8 mol/L~10-6 mol/L for 21 d significantly increased mineralized nodules.Exposu re of the cells to SIM at concentrations of 10-8~10-6 mol/L for 7d markedly increased the expression of TGF-β1,Smad2 and Smad3;and SIM at concentration of 10-7 mol/L produced the maximum effect,compare with the control group was statistically significantConclusionSIM could pormote the osteogenic differentiation of BMSCs,changes of the m RNA expression levels in TGF-β1/Smad signaling pathway might participate in this progress.