The Inhibition Mechanism Of Ginsenoside Rg3 On Invasive Activity Of Ovarian Cancer

Objectives: To observe the effect of ginsenoside Rg3 on invasion and metastasis of human ovarian cancer cell line SKOV3, and to further explore its mechanism. Methods: Different concentrations of ginsenoside Rg3 treatment of human ovarian cancer cell line SKOV3 in vitro, the proliferation of SKOV3 cells were detected by Transwell MTT assay, invasion of tumor cells, RT-PCR cells and Western blot method in the detection of VEGF, MMP-2 and MMP-9 protein content, immunofluorescence detection of NF- kappa B nuclear translocation. Results: Effect of ginsenoside Rg3 on SKOV3 cell, compared with the control group the absorbance(OD value) have no significant difference(P > 0.05), the inhibition rate were 2.98%, 4.47%, 7.46%. In 12.5, 25 and 50 mol/L of ginsenoside Rg3 has no obvious effect on the proliferation of SKOV3 cells; the invasion ability of SKOV3 cells by Transwell assay with different concentrations of ginsenoside Rg3 after 24 h treatment, the control group showed that there were 85.44 + 10.52 cells through the basement membrane, into the lower chamber; low dose Rg3 group was 55.28 + 9.17 a cell into thelower chamber; a middle dose of Rg3 group is 27.69 + 5.65 cells into the lowerchamber; high dose of Rg3 group is 6.21 + 2.46 cells into the lower chamber; RT-PCR and Western blot results showed: the expression of VEGF, MMP-2, MMP-9 is increase in control group, the expression is decrease in different concentrations ginsenoside Rg3 treatment(P<0.05), and with the increasing of Rg3 concentration,dose change; immunofluorescence results showed that: the control group showed strong nuclear staining, the expression of NF- B from the cytoplasm to the nucleus shift. Different concentrations of ginsenoside Rg3 after treatment, nuclear staining decreased significantly. Conclusions: Ginsenoside Rg3 can inhibit the invasion and metastasis of human ovarian cancer cells SKOV3, the mechanism is through inhibiting the expression of VEGF, MMP-2, MMP-9, inhibit the expression of NFkappa B.