Experimental Study On Fufangzhenhuaxiaoshen’an Capsule’s Effect To Experimental Diabetic Rats’ Podocyte’s Podocalyxin Expression

Purpose: In recent years on the pathogenesis of DN a lot of in-depth study and have confirmed that, Glomerular podocytes and its related molecules change plays a key role in the occurrence and development of DN. The experiment on the basis of The specialized research fund for the doctoral program of higher education-- From the adiponectin JieDuTongLuoBaoShen Capsule’s Intervention inflammation mechanism of diabetic nephropathy,through compound hazel spending renal capsule on experimental diabetic nephropathy rat Ann’s intervention, improve the podocyte mechanical barrier related the expression of protein molecules, by increasing the podocyte Podocalyxin protein expression of charge barrier to improve the stability and integrity, to achieve the sertoli cell protection.Method: With the chain urea with cephalosporins(STZ) induced Wistar rat model of DN, with urban bei sha Tanzania as positive control group, observation of traditional Chinese medicine compound hazel spending renal capsule of sertoli cell protection. Glucose determination using glucose meter and test paper measured blood sugar; Urea nitrogen(BUN) Serum creatinine(SCr) Total cholesterol(TC) Triglyceride(TG) using automatic biochemical analyzer test. The urine trace albumin ELISA determination respectively. HE staining and transmission electron microscope each rat kidney pathological changes, immunohistochemical method and rt-pcr detection of Podocalyxin in renal tissue homogenate protein expression.Result: Model group rats kidney index, urine, urine trace albumin, blood glucose, BUN, Scr, TC, TG were obviously higher in the normal group(P < 0.01),body weight, kidney Podocalyxin protein expression significantly lower than normal group(P < 0.01)。After the treatment,test compound hazel spending 12 weekend renal capsule low, middle, and high dose group and western medicine group of rat kidney index, urine, urine trace albumin, blood glucose, BUN, Scr, TC, TG is lower than the model group(P < 0.01),body weight, kidney podocytes Podocalyxin protein expression is higher than the model group(P < 0.01),renal pathological changes obviously improved。Conclusion: Fufangzhenhuaxiaoshenan Capsule for early diabetic nephropathy rats kidney plays a protective role, its mechanism may be related to its can improve the podocyte Podocalyxin protein expression。...