Establishment Of The MDA-MB-231-Fluc Cell Lines With Stable Luciferase Expression And Its Cytological Activities And Applications

Breast cancer is the malignancy that seriously threatens women’s health worldwide. The triple negative breast cancer(TNBC), as a subtype of malignant breast cancer, has high metastasis ability, poor prognosis, and high risk of relapse. A great deal of research attentions have been paid to this particular subtype of breast cancer. The MDA-MB-231 cell line is commonly used to study the TNBC. However, the disadvantage of this cell line is the lack of biomarkers for quantification and imaging in vivo. The firefly luciferase has been commonly used as the reporter to detect the specific cells or molecules. When it is integrated into the target genes, the luciferase gene can be stably expressed, without affecting the cell growth. In this thesis, we have successfully established the lentiviral-transfected MDA-MB-231-Fluc cell line, in which the luciferase gene is stably expressed, as judged by the genomic and m RNA assays. Then, by microscopic imaging, FCM and MTT assays, we confirmed that the MDA-MB-231-Fluc cell line possesses the same properties of cell morphology, cell cycle profile, and cell proliferation pattern as that of the MDA-MB-231 cells. Moreover, we implanted the MDA-MB-231-Fluc cell line into the BALB/c nude mice, and we determined that this cell line can be used to establish the breast cancer model. The luciferase gene expression was detected by light-emitting in the tumors generated from the implanted MDA-MB-231-Fluc cells. Overall, the MDA-MB-231-Fluc cell line we have established provided a convenient material for the study of triple negative breast cancer.