| Objective:We tested the rate of platelet-leukocyte surface glycoprotein of coronary heart disease UA blood stasis syndrome patients by using flow cytometry multi-labeled antibody, and detected the influences in injectable injection salvianolate and platelet-leukocyte surface glycoprotein of UA blood stasis syndrome. Clarify the drug injection improved the syndrome of UA by regulating platelet-leukocyte activation and aggregation feature and provide experimental evidence for salvianolate use in clinical and reveal the impact of modern biology of platelet-leukocyte adhesion, activation and aggregation in the molecular level. Provide some objective evidence for unstable angina syndrome type in TCM. Rely on modern medical research methods and quantitative criteria and classification of TCM to guide clinical treatment in the future.Method:1 Patients (n= 110) were chosen and divided into unstable angina group (n=90) and health group(n=20). All patients were given detection of expression level of CD62p on Platelet, CD162p on leukocyte membrane, CDllb on leukocyte and its subgroups, and aggregation rate of platelet-leukocyte with flow cytometry(FCM) and differences in FCM indexes were compared and analyzed within this 2 groups.2 Patients (n=110) were chosen and divided into unstable angina of blood stasis syndrome (BSS) group (BBS group, n=70) and unstable angina of non-BSS (NBBS group, n=20) group. All patients were given detection of expression level of CD62p on Platelet, CD162p on leukocyte membrane, CDllb on leukocyte and its subgroups, and aggregation rate of platelet-leukocyte with flow cytomery(FCM) and differences in FCM indexes were compared and analyzed within this 2 groups.3 Patients (n=65) with of blood stasis syndrome (BSS) were randomly divided into control group(n=33) and treatment group(n=32).The control group was treated for 10±2 days by Vinpocetine and routine medicine, while the treatment group was treated by salvianolate Injection and routine medicine. The results of clinical data in two groups were compared and analyze after treatment, which is the basis of clinical efficacy evaluation. All patients were given detection of expression level of CD62p on Platelet, CD162p on leukocyte membrane, CD11b on leukocyte and its subgroups, and aggregation rate of platelet-leukocyte with flow cytomery(FCM) and differences in FCM indexes were compared and analyzed within this 2 groups.4 All of the experiment data were statistically analyzed by SPSS20.0. Measurement data (a) was expressed by mean ±tandard deviation(x±s).Classification data was expressed by percentage (%).Counting data was expressed by frequency. Analyze the data which fits in normality (and homogeneity of variance) assumptions by ANOVA and T-test. P< 0.01 stands for extremely statistical significance, P< 0.05 stands for statistical significance, P> 0.05 stands for no statistical significance.Results: The study included 90 patients with UA,20 cases without disease. According to TCM syndrome type, these UA patients were classified, including 70 cases of blood stasis syndrome group,20 patients without blood stasis syndrome group. The acquired data were statistically analyzed, the two groups showed no significant difference in gender and age.1 The platelet CD62P expression level of UA group was significantly increased compared with the healthy group, the positive expression was significantly increased, the difference was highly significant statistically significant (P<0.01); the leukocyte membrane expression rate of CD162p in UA group were increased,compared with the healthy group, and the difference was statistically significant (P<0.05). The leukocyte membrane CD11b, MCD11b, NCD11b, LyCDl 1b expression levels of UA group patients were significantly increased, and its expressions were significantly higher than the healthy group. Among them,CDl 1b, MCD11b,NCD11b were statistically higher, the differences were very significant(P<0.01), and the difference of LyCD11b was statistically significant (P <0.05); the aggregation of PLA, PMA and PNA were significantly increased compared with healthy group(P<0.01).2 The comparison of blood stasis syndrome and non-blood stasis syndrome of coronary heart disease in flow results,the platelet CD62P expression level in patients with blood stasis syndrome in UA patients were significantly increased, the difference was highly significant statistically significant (P<0.01); the CDllb, MCD11b, NCD11b expression levels were significantly increased compared to non-blood stasis syndrome, there was statistically significant difference (P<0.05) groups; the aggregation rate of PLA, PNA, PMA were higher in patients with blood stasis than non-blood stasis patients, and there was very significant.(P<0.01).3 The evaluation of Salvia polyphenol acid injection in the treatment of patients with blood stasis syndrome of UA:Control group 9 cases (27.27%),effective in 18 cases (54.54%),5 cases (15.15%),1 case of aggravated (3.03%), the total efficiency was 81.18%.Treatment group 9 cases (28.12%),effective in 19cases (59.37%),4 cases (12.5%), increased 0 cases (0%), the total efficiency was 87.50%. There were no significantly differences between the two groups.(P> 0.05).4 The evaluation of Salvia polyphenol acid injection to electrocardiogram in the treatment of patients with blood stasis syndrome:Control group 6 cases (18.18%),effective in 16 cases (48.48%),lOcases (30.30%),l case of aggravated (3.03%), the total efficiency was 66.67%.Treatment group 6 cases (18.75%),effective in 17cases (53.12%),9 cases (28.15), increased 0 cases (0%), the total efficiency was 71.87%. There were no significantly differences between the two groups.(P> 0.05).5 The evaluation of Salvia polyphenol acid injection to the syndrome of blood stasis in the treatment of patients with blood stasis syndrome:Control group 7 cases (21.21%),effective in 19 cases (57.58%),6 cases (18.18%),1 case of aggravated (3.03%), the total efficiency was 81.81%.Treatment group 10 cases (31.25%),effective in 20cases (62.50%),2 cases (6.25%), increased 0 cases (0%), the total efficiency was 93.75%. There was significantly difference between the two groups in the total efficacy.(P> 0.05).6 The evaluation of Salvia polyphenol acid injection to the platelet, white blood cells, and platelets-leukocyte and sub-cluster collective aggregation:the treatment group and the control group compared with before treatment, the expression level of platelet CD62P were significantly reduced, the difference was highly statistically significant (P<0.01); the white membrane CD162p expression levels of the treatment group and the control group were lower than before treatment, the difference was statistically significant (P<0.05);The positive expression level of MCD11b in treatment group and control group patients was significantly lower than before treatment, the difference was statistically significant (P<0.05); the PLA, PMA, PNA aggregation rate in treatment group and the control group were significantly reduced than before treatment, PLA, PMA aggregation rate of decline has extremely statistically significant (P<0.01), PNA decline had significant difference (P<0.05); the PLyA aggregation rate of patients in treatment group was not statistically significant than control group(P> 0.05). But the two groups of flow cytometry in peripheral blood leukocytes, platelets had no significant difference between the groups (P> 0.05).Conclusion:1 UA patients platelet CD62p, leukocyte membrane CD11b, NCD11b, MCD11b, LyCDllb upregulate expression, leukocyte membrane CD162p, PLA aggregation rate were increased and aggregation of PLA, PMA, PNA increased had meaningful changes, but aggregation of PlyA rate did not make sense. Experiments show that platelet-leukocyte activation, aggregation are closely related to development of UA.2 The expression levels of CD62p, CD11b, NCD11b, MCDllb in patients with UA blood stasis syndrome group were increased and suggested that platelet activation, leukocyte activation associated with Blood; platelet-leukocyte aggregation rate were increased leukocyte aggregates, but the changes of PLyA had no significant, suggesting that the development of blood stasis syndrome and PLA, PMA, PlyA aggregation had correlation.3 Salvianolate injection had significant improvement on patients with UA blood stasis syndrome, suggesting that Salvianolate injection is one of the effective drugs for curing UA blood stasis syndrome.4 Salvianolate injection improved UA patients with blood stasis syndrome stasis syndrome manifestations, suggesting Salvianolate injection is one of the effective drugs for UA curing UA blood stasis syndrome.5 salvianolate injection decreased the level of CD62p, CD162p, CDllb on patient with UA blood stasis syndrome and reduce the PLA, PMA, PNA aggregation and inhibit platelet activation. Then reduce the inflammatory reaction, stable atherosclerotic plaque and reach the purpose of treating coronary heart disease UA with blood stasis syndrome. |
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