Effect Of Silencing Wnt10b Expression Stably On HepG2 Cells’ Proliferation, Cycle, Apoptosis, Migration And Invasion

Objective: Here we silenced Wnt10 b stably in HepG2 cells, observed and detected the changes of cellular proliferation, cell cycle, apoptosis, migration and invasion. Methods: We constructed the specific short hairpin RNA according to the Wnt10 b mRNA’s coded sequences, then stably silenced Wnt10 b in HepG2 via lentiviral vector, and detected Wnt10 b by Western blot. We observed the proliferation, cell cycle, apoptosis, migration and invasion of HepG2 by CCK-8 assay, colony formation assay, flow cytometry, Hoechst 33342 staining, Wound Healing assay, and transwell assay. Results: Western blotting results revealed that silencing Wnt10 b stably down-regulated the expression of Wnt10 b protein(P<0.05). Down-regulation of Wnt10 b expression distinctly suppressed proliferation, blocked cell cycle, and induced apoptosis in HepG2 cells(P<0.05). The ability of HepG2 cells’ migration and invasion in vitro were decreased significantly after silencing Wnt10 b stably(P<0.05). The interference target two shows more obvious changes of the cell growth characteristics than the interference target one according to our studies. Conclusion: Wnt10 b might play an important role in HepG2 cells’ proliferation, cycle, apoptosis, migration and invasion, indicating that Wnt10 b may be a novel and effective therapeutic target for preventing hepatocellular carcinoma.