| Objective: This study aimed to investigate the correlations between levels of mi R-196 a expression and clinical outcome in patients with advanced gastric cancer who received fluorouracil-based chemotherapy.Method:. FFPE samples prior to chemotherapy were collected from 61 patients receiving first-line fluorouracil-based chemotherapy in the oncology department of first people’s hospital of changzhou from Jan. 2010 to Dec. 2011. Total RNAs were extracted from normal and gastric tumor specimens. The levels of mi R-196 a were quantified by using real time q RT-PCR expression analysis. In addition, we will further analyze its clinical significance in gastric cancer.Results: The overall clinical benefit rate(CBR) of patients who treated with fluorouracil based regimens was 42.6% for advanced stage gastric cancer patients. The expression of mi R-196a(p=0.0006) was significantly overexpressed in gastric carcinoma tissues compare to adjacent tissue. The expression of mi R-196 a was also correlated to the sex and degree of cell differentiation. Patients with high level of mi R-196 a had lower CBR than patients with low level of mi R-196a(31.25% vs 46.7%,p=0.029).Kaplan-Meier survival analysis revealed that high level of mi R-196 a expression(Log rank test,HR=2.18,CI= 1.061 to 4.012,p=0.036) is closely associated with poor patient’s overall survival for patients with fluorouracil based regimens.Conclusion:.mi R-196 a hold great potential as prognostic biomarkers in late stage gastric cancer. |
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