The Detection Of RNA Concentration In The Cell Based On Circle Amplification

In the first chapter, this paper introduced the tumor, the stubborn disease, and its inducing factors. The tumor markers were discussed in detail, especially the microRNA. Also the paper introduced the nano materials used in the biochemical analysis. The paper described several analysis methods, such as fluorescence analysis and quartz crystal microbalance. We told about their theory and application. At last, it made a prospect about the detection of tumor markers.In the second chapter, we prepared GO. GO had the ability to absorb single nucleic acid and quench the close fluorescence group. It realized signal amplification through Klenow enzyme, and RNA was determined by fluorescence analysis. After amplification of klenow enzyme, the minimum detectable concentration reached 1.0 x 10-1 M, and the linear detection range of RNA was from 1.0× 10-1M to 1.0 ×10-9 M. Also, it realized detection for two kinds of RNA through different fluorescence group modified DNA terminal.In the last chapter, gold nanoparticles were synthesized. Crosslinking gold rubber nano clusters were obtained through the preparation of ammonia tree. Duplex-special nuclease was used, and it hydrolyzed the DNA hybridized with RNA. The RNA concentration was amplified and analyzed by QCM. the linear detection range of RNA was from 1.0 ×10-11 M to 1.0 ×10-9M. The working curve was mapped based on frequency change to RNA concentration. At last, the total RNA extraction of breast cancer cells were determined to detected the concentration of miR-203 RNA.