| In recent years, Nanoparticles (nanoparticles, NPs) has a very wide application inmedicine.The magnetic nanoparticles has many advantages such as magnetic targeting,superparamagnetic,and can be used MRI contrast agents, therefore it has beenwidespread concern in science.But there are some disadvatages,for example it is alsoeasy to gather, biocompatibility.The inorganic silica nanoparticle has stable structure,low toxicity and low immunogenicity,so using silica nanoparticle as surfacemodifications of magnetic nanoparticles should be carried out to increase the stabilityand biofunctionalityAtherosclerotic is one of the major causes of death in the western world.Theexpression of endothelial cell adhesion molecules VCAM-1will be increased at thesites of Atherosclerotic. So for the diagnosis and treatment of atherosclerosis, we canconsider making the drug or the carrier of specific targeted role in VCAM-1molecules.So we targeted nanoparticles could be prepared by coupling the ligands of VCAM-1molecules onto the nanoparticles surface.In this study, we used TEOS as silica source to cover Fe3O4nanoparticles,successfully synthesize core-shell nanopraticles of Fe3O4@SiO2by modified St bermethod. At the same time, fluorescent FTIC was doped in the interior of thenanoparticles. It not only has the magnetic targeting property, be used as MRI contrastagents, but also the fluorescence imaging characteristics. Through coupling APTMS wefurther functionalized the nanoparticle surfaces. As an atherosclerotic targeting ligand,anti-VCAM-1was covalently bonded to the surface of the nanoparticles. The silica isquite biocompatible and resistant to biodegradation. Subsequently, the nanoparticles canbe uesd for fluorescence labeling, targeting drag delivery and MRI contrast agents. It isconcluded that these silica nanoparticles have high potential in the diagnosis andtreatment of atherosclerosise. |
