Preliminary Exploration About Effect Of Atmospheric Particulate Pollution On Human Breast Cancer In Shandong Province

Background:With the advance of urbanization and industrialization, the release of toxic air pollutants is prevalent in China, as well as many cancers including breast cancer. Among women, breast cancer is the most frequently diagnosed malignant tumor in China. Thus, it is important to understand whether and how exposure to air pollution has adverse effects on breast cancers.Methods:Ambient air information was obtained from Environmental Quality Statement issued by Environmental Protection Bureau. We evaluated the association between atmospheric particulate levels and characteristics of human breast cancer via Multivariate Logistic Regression Model. Single nucleotide polymorphism was detected via TaqMan-based Real-Time PCR and direct sequencing. Stratified analysis and multivariate logistic regression model were adopted to analyze the association between polymorphism and breast cancers. We further explored the way how this polymorphism impacts the development of human breast cancer, via tissue RNA isolation, quantitative reverse-transcription PCR, real-time PCR, cell culture and transfection, invasion and migration assay.Results:Long-term particulate exposure contributes to the development of estrogen-receptor positive breast cancer. Distribution of microRNA-27a polymorphism is related to local levels of atmospheric particulate, and this polymorphism is associated with reduced breast cancer risk and able to decrease the expression of microRNA-27a in human breast cancers tissues.Conclusion:Our findings indicate that long-term atmospheric particulate exposure may contribute to the development of estrogen-dependent breast cancer by playing the role of a xenoestrogen, and also provides insight that atmospheric particulate pollution and gene polymorphisms may function together as regulators of breast cancers. Furthermore, it is urgently necessary to study the association between air pollution and breast cancer to improve the living quality and health of females, and applicable public health strategies may need to be established or modified as soon as possible.