| In this paper, the chemical components of Fructus Akebia were researched. The main contents are listed as follows:1. For provide the reference for the industrialized production, the optimal study on extraction technology of Fufang Changtai granules by orthogonal experimental design. The orthogonal test was employed taking the content of ginsenoside Rb1, hederagenin and the amount of solids as indexes to investigate the influences on extraction results and the influences on extraction results of the amount of water, extraction times and times of decoction were investigated. The content of ginsenoside Rb1 and hederagenin was determined by HPLC. The mobile phase was acetonitrile(A)-water(B) and gradient elution as follow: 0~5 min, 10%~30%A; 5~20 min, 30%~45%A; 20~22 min,45%~10% A. Optimum extraction technology was: refluxing extracted 3 times with 8 times of the amount of water with 1 h each time. Under above conditions ginsenoside Rb1 and hederagenin were 2.48 mg·g-1,10.50 mg·g-1 respectively and the extraction rate of extractum was 16.72%. The optimum extraction technology was highly reproducible, stable and suitable for industrialized production of Fufang Changtai granules.2. This research was completed to analyze and identify the chemical components in water extract of Fruetus Akebiae by UPLC-ESI-Q-TOF-MS. The analysis was performed on an ACQUITY HSS T3 reverse phase column(2.1 mm × 100 mm, 1.8 μm). The mobile phase consisted of acetonitrile and 0.1% formic acid was used for gradient elution, the flow rate was 0.4 m L min-1. Quadruple-time of flight-mass spectrometry was applied for qualitative analysis under positive and negative ion mode with ESI ion source was used for mass spectra. The results indicated that sixteen compounds from water extract of Fruetus Akebiae had been identified by direct comparison in both positive and negative ion mass data, the element compositions analysis and the data of the literature.3. HPLC-ELSD was used to establish a quantitative determination method for hederagenin in Fructus Akebia after being hydrolyzed, The optimum conditions were as follows: Samples were analyzed with Allitima C18 column( 4.6 mm × 250 mm, 5 μm) and acetonitrile-0.1 % formic acid solution was used as mobile phases, flow rate was 1.0 m L·min-1, column temperature of 35 ℃, drift tube temperature of ELSD was set at 90 ℃, and flow rate of nitrogen was maintained 2.5 L·min-1. There were good linear relationship between natural logarithm of peak areas and injection quality at the range of 0.36~3.6 μg( r=0.9994). The average recovery of hederagenin was 99.04 % and RSD was 2.0 %.The method is simple, accurate and specific. It provides a reliable way for evaluating the quality of Fructus Akebiae. The study provides the scientific evidence for using Fructus Akebiae.4. In this paper, an HPLC-ELSD method for determination of hederagenin in Fruetus Akebiae was established and the content of hederagenin in Fructus Akebiae different parts was compared. The contents of hederagenin in fruit, seed and peel were 24.28 mg/g~35.18 mg·g-1, 37.21 mg/g~58.38 mg·g-1, 7.83 mg/g~19.44 mg·g-1, respectively. The results showed the contents of hederagenin in different medicinal parts of Fructus Akebiae were significantly different. The method was simple, stable, reliable and reproducible. |
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