Effects Of Wnt-β/Catein Signal Pathway Huatan Tongyu Jiedu Decoction On The Mechansim Of Invasion And Metastasis Of SGC-7901 And MKN-45 In Human Gastric Cancer Cells

BackgroundStomach cancer (carcinoma of stomach) is one of common malignant tumors in China, because of its high mortality and become a serious threat to the health of esidents of the disease. In the treatment of gastric cancer, the traditional surgery is still he preferred treatment, but treatment is low in patients with early gastric cancer, yet ost the best timing of surgery. Cancer chemotherapy and radiotherapy is an important idjunct to gastric cancer, chemotherapy and radiotherapy with its severe suppression of he immune function of cancer patients and bone marrow suppression restricted its clinical application.Molecular targeted therapy show, Wnt/β-catein signaling pathway involved in many umor cells, intercellular adhesion, protease expression, angiogenesis and tumor cell nvasion and metastasis. The issue with the compound Huatan Tongyu Jiedu Decoction raditional water extract for the study, research phlegm stasis detoxification side effects On human gastric cancer cell SGC-7901 and MKN-45 mechanism of invasion and netastasis.ObjectiveFor investigating the anti-invasive and anti-metastatic mechanisms of Huatan fongyu Jiedu Decoction treatment on human middle-grade gastric adenocarcinoma cell ine SGC-7901 and human low-grade gastric adenocarcinoma cell line MKN-45, we checked on the expression of LiCl-induced Wnt/β-catenin pathway targets VEGF, COX-2 and regulatory molecules β-catenin, glycogen synthase kinase-3β, the ranscription factor T-cell factor 4(TCF4) in those two gastric adenocarcinoma cell.MethodFor water extraction of Huatan Tongyu Jiedu Decoction,the optimum extraction precess was chosen out..Dried powders were dissolved in distilled water, filtered and treated on human gastric adenocarcinoma cell line SGC-7901/MKN-45.Cell viability of cell proliferation was determined by MTT assay after 24 h of exposure.The absorbance was measured to analysis the inhibition ratio; Western blot were employed to evaluate various concentrations of Huatan Tongyu Jiedu Decoction adminstration on the expression level of β-catenin、p-GSK、TCF-4、VEGF、COX-2 and nuclear β-catenin in LiCl-stimulated SGC-7901 and MKN-45 cells.Result1 The optimum extraction precess of Huatan Tongyu Jiedu Decoction was Soaked in he water for 40 mins and then boiled thrice, each for 45 mins in distilled water.with 10BV.2.Compared to LiCl control group,the Human gastric adenocarcinoma cell line MKN-45 with various concertrations of Huatan Tongyu Jiedu Decoction ; 5,10,20,40,80μg/ml) treatment for 24h showed that RPTS had powerful proliferation ;ffect in a dose-dependent maner(p<0.05).3 Compared to LiCl control group,Huatan Tongyu Jiedu Decoction,in a dose-dependent nanner,significantly reduced the expression of VEGF、COX-2 at the protein evel(p<0.05) while the level of β-catenin、p-GSK、nuclear β-catenin、TCF-4 in SGC-7901 and MKN-45.