Effect Of Marine Oral Liquid In Type 2 Diabetic Rats

ObjectivePreparation type 2 diabetic rat model by high fat feed combined with streptozocin (STZ) inducing, and then study the effect of Marine Oral Liquid(HS) on type 2 diabetic rats, to explore its mechanism.Methods(一)Model establishment methodThe SD rats were divided into control group and model group, Control group fed with ordinary feed, model group fed with high fat feed.after four weeks, model group was intraperitoneally injected 1%STZ solution according to the 40mg kg-1, and normal control group intraperitoneal injection of an equal volume of citric acid sodium citrate buffer solution.72h after modeling, the diabetic rats with fastig blood glucose levels higher than 11. lmmol·L-1 were selected.(二)Grouping and administration methodThe diabetic rats were divided into 5 groups:diabetic model, HS low dose (2.5mL·kg-1·d-1), HS middle dose(5. OmL·kg-1·d-1).HS high dose(10. OmL ·kg-1·d-1) and rosiglitazone(2mg·kg-1·d-1)’, in addition,10 normal SD rats were used as normal group, normal rats and diabetic control rats received the same volume of distilled water. Control group fed with ordinary feed and other groups rat fed with high fat feed during the dosing period(三)Measurement method① Measurement of general index and pathological morphologyObservated animals status regularly, weighted and recorded the animal body weight every weeks; after the last administration, testing 24h feeding, drinking water and urine circumstances; the liver and kidneys index were been calculated, HE staining pancreas and kedneys, and then photographed under a microscope.② Effect of HS on metabolism of glucose and lipid levels in type 2 diabetic ratsMeasurement the fasting blood glucose by glucose monitors at 0、2、 4 weeks after administration, and levels of fasting glucose in the tolerance testing, detect the content of glycosylated hemoglobin, hepaticglycogen and FFA by kits;detect the content of TC、TG、LDL-C、 HDL-C in serum by automatic biochemical analyzer.③ Study on the effection mechanism of HS in type 2 diabetic ratsDetect the content of FINS in serum by kits after the last administration, and then calculation the ISI and HOMA-IRI;measure the expression of IRS-2 and PTP-1BmRNA in liver by RT-PCR.Results(一)Result after modellingDuring 4 weeks high fat feed molding, animals generally status all good, Compared with the normal group, body weight of model group were significantly higher since second weeks (P< 0.05); 72h after intraperitoneal injection of STZ modeling, the fasting blood glucose of model group were significantly than the normal group(P< 0.05), suggesting that model of the type 2 diabetes mellitus were built successfully.(二)Result of general indexes and pathologicalCompared with the model group,3 weeks after administration, the body weight of low-dose group increased significantly (P< 0.05); the body weight and kidney index of the low and middle dose groups increased significantly when 4 weeks after administration (P< 0.05); 24h feeding of high dose group were significantly decreased (P< 0.05); 24h drinking water volume of middle and high dose group decreased significantly (P < 0.05); each dose group were significantly decreased in liver index (P < 0.01 or 0.05), the pathological morphology of pancreatic, kidney tissue were significantly improved in each group.(三)Effect of HS on metabolism of glucose and lipid levels in type 2 diabetic ratsCompared with the model group, the fasting blood glucose of low and high dose groups were significantly lower after administered 2 weeks, the fasting blood glucose in the glucose tolerance test of each time point、 levels of AUC、the content of glycated hemoglobin level after administered 4 weeks of the each dose group were decreased significantly, hepatic glycogen also significantly increased in middle dose and high groups(P< 0.05); TG, TC levels decreased significantly about all dose of the HS group (P<0.01); low, medium dose group LDL-C, FFA was significantly decreased (P<0.05);HDL-C of the middle dose group HDL-C significantly increased (P< 0.05).(四)Study on the effection mechanism of HS in type 2 diabetic ratsCompared with the model group, high dose group could significantly increase the insulin levels (P< 0.01); low and high dose groups could significantly enhance insulin sensitivity (P< 0.05); the expression of insulin resistance and PTP-1B mRNA (P<0.01) decreased significantly in each group, high dose group significantly to improve the expressionof IRS-2 mRNA (P<0.01).ConclusionIn summary, HS has obvious effects on type 2 diabetic rats, probably by regulating the level of glucose and lipid metabolism and IRS-1mRNA, PTP-1BmRNA expression level.