The Study Of MicroRNA As A Predictor In The Early Stage Of Acute Pancreatitis

bjective: Acute pancreatitis is a common disease of the digestive system which can be divided into severe acute pancreatitis, moderately severe acute pancreatitis and mild acute pancreatitis according to the latest Atlanta standards. It is obvious different in therapeutic strategies and prognosis with these different types. MAP is mild with lower mortality and good prognosis and the pancreas is often manifested in edema,on the other hand SAP has a larger changes in condition with local or systemic associated complications and poor prognosis,it has a mortality rate of 36-50 %.Pancreatic pathology often showed more hemorrhagic necrosis. It is very important for the early prediction of severe acute pancreatitis.The predictors of acute pancreatitis include scoring systems such as APACHE Ⅱ scores Ranson scores BISAP scores,Clinically relevant indicators such as body mass index acute fluid collection,Imaging index such as ultrasound and CT, laboratory indicators such as C-Reactive Protein,triggering receptor expressed on myeloid cells-1,PCT and B-cell activating factor of TNF family. These predictors both have their advantages and disadvantages in the early predicting of AP. Micro RNA is a non-coding RNA with 20-22 nt length, which is from a length of single-stranded RNA having a hairpin precursor after enzyme Drosha and Dicer enzyme shear generation. Mi RNA by 3 ’non-coding region and the target m RNA molecule(3’-UTR) complementary mating, translation of the target m RNA molecule is inhibited or cause-specific cleavage of m RNA molecules, resulting in post-transcriptional level of the target gene expression regulation.Mi RNA involved in many physiological processes of the body. A large number of studies have found, micro RNA closely related with inflammation. This study was to explore the mi R-21, mi R-146 a, mi R-26 a, mi R-200 a, mi R-10 b changes in rats with acute pancreatitis and its clinical significance.Methods : 36 SPF SD rats were randomly divided into three groups, necrotizing pancreatitis group, edematous pancreatitis group and control groups. The model of necrotizing pancreatitis group and edematous pancreatitis group was prepared by laparotomy retrograde injection of 5% and 1% sodium taurocholate,while the model in the control group was dealde with turning pancreas.Detecting respectively mi R-21, mi R-146 a, mi R-26 a, mi R-200 a, mi R-10 b changes the in three groups of rats plasma as well as serum amylase, lipase, IL-1 and IL-6 in the 12 and 24 hours after modeling. The rat pancreatic tissue were taken to stain with Hematoxylin and eosin and make pancreatic pathology score in the 12 and 24 hours after modeling. The expression of PTEN in the pancreas was detected by immunohistochemical and the serum levels of NF-KB was detected by ELISA.Results: The levels of amylase, lipase, IL-1, IL-6 in necrotizing pancreatitis group and edematous pancreatitis group was higher than the control group at the same point, the difference was statistically significant(P <0.05). The difference of serum amylase in hemorrhagic necrotizing pancreatitis group and edematous pancreatitis group was not statistically significant. The levels of serum lipase, IL-1 and IL-6 in necrotizing pancreatitis group was higher than the edematous pancreatitis group,the difference was statistically significant(P <0.05). At the same time point pancreatic pathology score of necrotizing pancreatitis group was higher than the score of edematous pancreatitis group and the control group and the difference was statistically significant(P<0.05).The difference of pancreatic pathology score between edematous pancreatitis group and the control group was not statistically significant.The level of mi R-21 in hemorrhagic necrotizing pancreatitis group was significantly lower than edematous pancreatitis group and the control group, the difference was statistically significant(P <0.05). The level of mi R-21 in edematous pancreatitis group and the control group was no statisticaldifferent. The level of mi R-146 a, mi R-26 a, mi R-200 a, mi R-10 b, mi R-21 in hemorrhagic pancreatitis group, edematous pancreatitis and control group was not statistically different. At the same time point,The expression of PTEN in hemorrhagic pancreatitis group was higher than edematous pancreatitis group and the control group, the difference was statistically significant(P <0.05).The differences of PTEN expression between edematous pancreatitis and control groups was not statistically significant. The level of NF-KB in hemorrhagic pancreatitis group was higher than edematous pancreatitis group and the control group, the difference was statistically significant(P <0.05), NF-KB content between edematous pancreatitis and control groups showed no statistical differences.Conclusion:Mi R-21 may become a predictor of acute pancreatitis in the early stage.Mi R-21 and PTEN may be important in acute pancreatitis.