The Expression Of TL-17F On Gastric Carcinoma And The Effect Of TL-17F On Gastric Cell Biological Characteristics

Objective:Our study aims at examing the expression of IL-17 F in human gastric cancer and exploreing the role of IL-17 F plays in the progress of gastric cancer and its mechanism.Methods:1 Western-blot and RT-PCR were used to evaluate the expression of IL-17 F in gastric cancer tissue and normal tissue and analysis the correlation between gastric cancer and pathological parameters2 MTT assay: To determine the effect of IL-17 F on SGC7901 cell proliferation activity with different concentrations( 10ng/ml 、 20ng/ml 、30ng/ml、40ng/ml、50ng/ml、100ng/ml).3 RT-PCR and western-blot were performed to evaluate the effect of m RNA and protein expression of IL-17 R in SGC7901 cells after si RNA transfecton.4 Western-blot was performed to detected the expression of P-STAT3 of tansfection group, control transfection group treated by IL-17 F with silence IL-17 R or with TPL2 inhibited.5 The wound scrape assay and transwell experiments were used to detect the SGC7901 cells ability of migration and invasion of tansfection group, control transfection group treated by IL-17 F with silence IL-17 R or with TPL2 inhibited.6 ELISA technology was used to analyze the levels of IL-6, IL-8 and VEGF in culture systems of SGC7901 of tansfection group treated by IL-17 F with silence IL-17 R or with TPL2 inhibited.Results:1 The RT-PCR show that compared to normal tissue, the expression of IL-17 F, VEGF and IL-6m RNA in gastric cancer tissue was significantly increased and the expression levels of IL-17 Fm RNA was significantly correlated with inflammatory factor VEGF,IL-6m RNA.2 The western-blot show that compared to normal tissue, the expression of IL-17 F protein in gastric cancer tissue was significantly increased.3 Correlation analysis between gastric cancer tissues of IL-17 F m RNA and protein expression in gastric cancer patients with and pathological parameters: gastric cancer tissues IL-17 F m RNA and protein in expression levels in relevant with TNM stage, with the patient’s, age, gender, independent of the tumor size.4 MTT results showed that there was no significant change in cell relative proliferation rate with different concentrations of IL-17 F, the difference was not statistically significant(P>0.05).5 To determine the efficiency of RNA interference, we first analyzed the levels of IL-17 R gene expression in the IL-17 R si RNA and control si RNA groups. There was markedly decreased gene expression after transfection of IL-17 R si RNA compared with transfection in the control si NRA group.6 The effect of IL-17 F on the migration of SGC7901 cells of silence IL-17 R.The wound scrap assay show that compared to control transfection, si RNA inhibition of IL-17 R significantly decreased SGC7901 cells migration promoted by IL-17 F in the wound scrape model.7 The effect of IL-17 F on the invasion of SGC7901 cells of silence IL-17RThe transwell experiments show that compared to transfection group, the number of invaded SGC7901 cells of control transfection group was significantly increased. There was significant distinguish between transfection and control transfenction group treated with IL-17 F.8 The effect of IL-17 F on the activation of STAT3 of SGC7901 cells of silence IL-17 R.The western-blot experiments show that compared to transfection group, the activation of STAT3 of control transfection group was significantly increased. There was significant distinguish between transfection and control transfenction group treated with IL-17 F.9 The effect of IL-17 F on the production of cytokines of SGC7901 cells of silence IL-17RThe ELISA experiments show that compared to transfection group, the production of cytokines of SGC7901 cells of control transfection group was significantly increased. There was significant distinguish between transfection and control transfenction group treated with IL-17 F.10 The effect of IL-17 F on the migration of SGC7901 cells with TPL2 inhibited.The wound scrap assay show that compared to control group, the migration of SGC7901 cells was significantly decreased by IL-17 F after treated with TKI.11 The effect of IL-17 F on the invasion of SGC7901 cells with TPL2 inhibited.The transwell experiments show that compared to control group, the number of invaded SGC7901 cells was significantly decreased by IL-17 F after treated with TKI.12 The effect of IL-17 F on the activation of STAT3 of SGC7901 cells with TPL2 inhibited.The western-blot show that compared to control group, the activation of STAT3 was significantly decreased by IL-17 F after treated with TKI.13 The effect of IL-17 F on the production of cytokines of SGC7901 cells with TPL2 inhibited.The ELISA show that compared to control group, the production of cytokines of IL-6, IL-8 and VEGF were significantly decreased by IL-17 F after treated with TKI.Conclusions:1 Compared to normal tissue, the expression of IL-17 F in gastric cancer tissue was significantly increased and in relevant with TNM stage indicating that IL-17 F may promote gastric cancer progression.2 The gastric carcinoma IL-17 F in expression levels of inflammatory factor VEGF, IL-6 expression was significantly correlated indicating that IL-17 F may promote gastric cancer progression by up-regulating the expression of IL-6 and VEGF.3 IL-17 F promote gastric cancer progression by acting the pathway of TPL2- STAT3 to enhance the ability of SGC7901 cells of migration, invasion and secretion of IL-6, IL-8, VEGF through IL-17 R.