Effect Of Salt Stress On The Adhesive Ability Of Helicobacter Pylori And Expression Of Adhesion Related Genes

AimsHelicobacter pylori (H.pylori) is one of the most common Gram negative bacteria in the world. H.pylori can produce CagA protein that can enter into the gastric mucosal cells via T4SS. Previous studies indicated that high salt induced more CagA to enter into cells. The objective of our research is to investigate the effect of salt stress on H.pylori adhesive ability and adhesion-related genes expression.MethodsH.pylori strain 11637 was culture on the special solid medium, and the morphology of H.pylori was observed under microscope after Gram staining. Total RNA was isolated with TRIzol, and qRT-PCR was performed to evaluate the expressions level of babA2、sabA、alpA/B、oipA、 hopZ at different time points and salt concentrations. The method developed by Skindersoe and colleagues was used to test the adhesive ability of H.pylori. SPSS 19 was used for statistical analysis.ResultsSalt stress inhibited growth of H.pylori, and spherical-shaped bacterium was more frequent under high salt concentration. Results of qRT-PCR indicated that expressions of babA2、sabA、 alpA/B、oipA、hopZ were all increased at 48 hour (P<0.05), but there were no significant difference between 24 and 72 hour groups. So we use bacterium culltured for 48 hour to detect adhesive capacity of H.pylori to SGC-7901. Result suggest that neither salt concentrations nor different MOIs of H.pylori show significant impact on the adhesive ability of H.pylori to SGC-7901.ConclusionSalt stress could inhibit growth of bacterium and induce more spherical-shaped H.pylori. babA2、sabA、alpA/B、oipA、hopZ expression increased along with salt concentration at 48 hour, but there was no effect on adhesive ability of H.pylori.