The Change And Interaction Of IL-21 And Tc1 Cell In Perigheral Blood And Lung Lesion Of Nsclc

Object To explore the change and interaction of interleukin-21(IL-21) and CD8+ IFN- γ +T(Tc1)cell in perigheral blood (PB) and corresponding lung cancer and para-cancer tissues of the non small cell lung cancer (NSCLC) patients.Methods The samples of PB、lung cancer and para-cancer tissues was collected from 30 NSCLC inpatients, and the PB of 16 healthy people was control group. The concentration of IL-21 in the serum and tissue homogenates was tested by ELISA kit and the proportion of CD8+IL-21R+T and Tcl cell from above samples was determined by flow cytometry; CD8+T cells were obtained from peripheral blood mononuclear cells (PBMCs) of the patients using human T-cell isolation kit, purified T cells were cultured with rhIL-21 or no rhIL-21, co-cultured group and control group ,after 24h and 48h of culture, the percentage of Tel cell was calculated by flow cytometry. Last the relationship of the parameter was evaluated.Results The concentration of IL-21 in the serum from patients was significantly higher as compared to the control group[(23.25±4.50) pg/ml VS (3.16±1.46) pg/ml (P<0.05)],the proportion of CD8+IL-21R+T and Tcl cell from patients PB was significantly lower compared with the control group, respectively [(1.51±0.32)%VS (2.92±1.08)%(P<0.05)、 (11.42±2.82) %VS (33.86±10.19) % (P<0.05)]. As well as the concentration of IL-21 from tissue homogenates[(36.38±7.91) pg/ml VS (14.91±4.11) pg/ml (P<0.05)],the percentage of CD8+IL-21R+T and Tel cell from lung cancer tissues was significantly increase compared with the corresponding para-lung cancer tissues ,respectively[(1.35±0.33)%VS (1.22±0.27)%(P<0.05)、(5.49±1.20)%VS (3.87±0.66)% (P<0.05)].The frequency of Tcl from both patients PB and lung cancer tissues had a positive correlation with CD8+IL-21R+T cell, respectively(r=0.607,r=0.505, both P<0.05).The concentration of IL-21 in serum from patients and tissue homogenates of lung lesion and the frequency of Tel 、CD8+IL-21R+T cell in patients PB and lung lesion both had the differentiation in different TNM phase ,and had the downtrend with the development of the disease. After 24h and 48h of culture with rhIL-21, the frequencies of Tel in purified CD8+T cells from co-cultured group were both higher than control group, both P<0.05.Conclusion In PB and lung lesion of the NSCLC patients, the content of IL-21 was increasing and downtrend with the developing of the disease; the CD8+IL-21R+T and Tel cell from patients PB was down-regulation and recruitment into lung cancer lesion, and then had downtrend with the advance of the disease; The frequency of Tcl from both patients PB and lung cancer tissues had a positive correlation with CD8+IL-21R+T cell; IL-21 may combine with CD8+IL-21R and then promote CD8+T cell secret the IFN-γ, implement the anti-tumor effect.