| Background Esophageal cancer is one of the world’s most common gastrointestinal cancer, the most common histological type is squamous cell carcinoma. Previous studies have found that the mechanism of esophageal cancer is a multi-step, multi-factor, integrated multi-gene mutation accumulation process, but its complex pathophysiology and genetic characteristics of the many needs further exploration. IL-23 is a novel heterodimeric inflammatory cytokine Oppmann found. The latest study found that, IL-23 in the tumor microenvironment significantly overexpressed suppress CD8+T cells involved in tumor invasion started occurrence; can also be regulated by the expression of MMP-9 in the role of Th17 cells infiltrate the recruitment of inflammatory cells, and then promote angiogenesis, plays an important role in the process of tumor invasion and metastasis. By Th17 cells role in the recruitment of inflammatory cells infiltration to increase the expression of MMP-9 play an important role in the development of tumors. The MMP-9 is one of the most important matrix metalloproteinases degrade extracellular matrix, the rapid proliferation of cancer cells, invasion and metastasis in the process has a very important role. However, in the case of esophageal squamous cell carcinoma and its relation to the development of IL-23 and tumor incidence;Relationship Invasion IL-23 and MMP-9 expression correlated with esophageal squamous cell carcinoma is not yet reported.Objective In this study, real-time PCR detection of esophageal squamous cell carcinoma, the expression of IL-23 by double-antibody sandwich ELISA serum IL-23 and MMP-9 expression and analysis of the correlation between the two, To investigate the role of IL-23 in the pathogenesis of invasion and metastasis of esophageal squamous cell carcinoma, and lay the theoretical foundation for elucidating the role of IL-23 in tumor formation and metastasis.Methods Collected in 2012-10 to 2014-3, the First Affiliated Hospital of Guangxi Medical University, I cardiothoracic surgery and hospitalization pathologically diagnosed 48 patients with esophageal squamous cell carcinoma, patients did not receive radiotherapy and chemotherapy. Also collect the same period in our hospital electronic gastroscope examination benign biopsy results in 30 cases in the control group, no other parts of the primary tumor and metastases. Specimens have been patients and their families to obtain consent and ethics committee approval. Real-time PCR detection of esophageal squamous cell carcinoma, the expression of IL-23 by double-antibody sandwich ELISA serum assay of IL-23 and MMP-9 expression and analysis of the correlation between using SPSS 16.0 statistical software to analyze the relationship between IL-23 expression in the case of a patient with the clinical and pathological features and the relevance of serum IL-23 and MMP-9 expression.Results The relative expression of IL-23 gene in squamous cell carcinoma and adjacent tissues were 8.72 ± 2.14 and 2.39 ± 0.78, the difference was statistically significant (t= 3.52, P<0.05), IL-23 gene in ESCC tissues regulated.48 cases of esophageal squamous cell carcinoma patients by age, gender, ethnic and TNM stage, lymph node metastasis group. IL-23 expression and lymph node metastasis-related genes, P= 0.028; age, sex TNM stage ,, ethnic (Han and Zhuang) is independent (P> 0.05).Squamous cell carcinoma group and control group, serum IL-23 expression levels were (123.63 ± 18.08pg/mL,26.66 ± 8.17 pg/mL), the difference was statistically significant (t 26.66. P<0.05). The serum levels of MMP-9 expression was 177.38 ± 34.71 ng/mL and 39.17 ± 9.63ng/mL, the difference was statistically significant (t= 16.89.P<0.05).48 cases of esophageal squamous cell carcinoma serum IL-23 expression and TNM stage, lymph node metastasis and differentiation (highly differentiated and poorly differentiated) related, P values were 0.040,0.042 and 0.038; gender, age, ethnicity (Han and Zhuang) is independent, P> 0.05. Pearson correlation analysis showed, IL-23 and MMP 9-expression in the serum of patients with esophageal squamous cell carcinoma were positively correlated (r= 0.790, P <0.05). Both the control group showed no correlation (r= 0.140, P> 0.05).Conclusion1, The high expression of IL-23 may be closely related to esophageal squamous cell carcinoma, progression and metastasis.2, IL-23 possibly through upregulation of MMP-9 to participate in the invasion and metastasis of esophageal squamous cell carcinoma. |
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