The Effect Of Perindopril On 3-mercaptopyruvate Sulfurtransferase/hydrogen Sulfide Biosynthentic Pathway In Isoprenaline Induced Cardiac Hypertrophy Rats

Background: Cardiac hypertrophy is an independent risk factor. And it can lead to the incidence of cardiovascular disease and mortality increased significantly. It is also with heart disease such as hypertension, coronary atherosclerotic heart disease, arrhythmias,sudden cardiac death and other cardiovascular diseases in close relationships.Angiotensin converting enzyme inhibitors(ACEI) are often used in the treatment of cardiac hypertrophy-related diseases as it could clearly reduce blood pressure and improve ventricular remodeling. Some studies have found ACEI can affect the hydrogen sulfide content of myocardial tissue in cardiac hypertrophy rats caused by aortic coarctation, spontaneously hypertensive or even normal rats. Physiological concentrations of H2 S is the third endogenous gas signaling molecule which was discovered after nitric oxide(CO), nitric oxide(NO). H2 S synthesis of myocardial tissue mainly through cystathionine-γ-lyase(CSE) and 3-mercaptopyruvate sulfurtransferase(3MST) in mammals. Numerous studies have shown that many in the development of cardiovascular disease and treatment process, the H2 S content in myocardial tissue will be changed at the same time.Objective: To study how perindopril affect the 3MST/H2 S synthesis pathway of cardiac hypertrophy myocardial tissue in the treatment of isoproterenol(isoprenaline,ISO) induced rats.Methods: 60 rats were randomly divided into six groups: sodium hydrosulfide +model group(DNP), perindopril group(COP), high doses of perindopril + model group(DHP), low doses of perindopril + model group(DLP), cardiac hypertrophy model group(DIC) and control group(CON). Cardiac hypertrophy model was prepared by multi-point subcutaneously ISO injection, then high and low doses of perindopril or sodium hydrosulfide intraperitoneal injection solution were given in different constituencies. Detectting the rat heart mass index(HMI) and left ventricular mass index(LVWI), cardiac pathology, the hydrogen sulfide content and 3MST expression.Results: Comparing with the CON, HMI and LVMI increased, with varying degrees of myocardial hypertrophy, the content of H2 S decreased and 3MST decreased expression in four groups of cardiac hypertrophy model rats, however in COP, HMI and LVMI was no significant difference and without cardiac hypertrophy, but the content of myocardial H2 S increased, and also as 3MST expressed. Comparing with the DIC,HMI and LVMI decreased, the content of myocardial H2 S and 3MST expression also increased in DHP, DLP and DNP. Comparing with the DHP, HMI and LVMI increased the content of myocardial H2 S decreased, 3MST decreased expression in DLP.Conclusion: Multi subcutaneous injection of isoprenaline in rats could cause significant cardiac hypertrophy, perindopril could treat cardiac hypertrophy with a clear effect in this model. Perindopril could increase the content of myocardial H2 S and3MST expression in the treatment of cardiac hypertrophy induced by isoprotenaline.Within a certain dose range, the effect of perindopril treatment, high-dose(4 mg/kg/d)was superior to low-dose(2 mg/kg/d). Exogenous sodium hydrosulfide could not only prove a certain extent, the treatment of cardiac hypertrophy in rats induced by isoprote,but also increase the content of hydrogen sulfide in myocardial tissue and 3MST expression as a exogenous hydrogen sulfide donor. Perindopril was closely related with3MST/ H2 S biosynthentic pathway in the treatment of myocardial hypertrophy, and sodium hydrosulfide also had a similar effect as exogenous hydrogen sulfide donor,and it suggested that the close relationship between ACEI and H2 S.