| Objective: Hepatocellular carcinoma is one of the most common malignant cancer, Hereinafter referred to as live cancer. For the sixth of all the total number of cancers. More than half of liver cancer worldwide occur in china, The mortality of liver cancer is high, ranking the 2nd place in world cancer. At present the treatment of liver cancer, including surgery, radiation and chemotherapy, interventional therapy, radiofrequency ablation, biological immune, etc. Surgery is the preferred treatment, But its specificity for liver cancer, a few patients have the chance to surgery. After the operation, the recurrence of liver cancer is high. For liver cancer, chemotherapy has certain curative effect. But because of its side effects, patients can’t tolerate or short of effective dose. Other treatments can’t improve obviously for the overall survival of patients with liver cancer. As a new eye-catching method, photodynamic therapy has achieved good results in the treatment of malignant tumor, with the advantages of minimally invasive and little side effect. At home and abroad, photodynamic as a curative effect in treatment of liver cancer has be confirmed by a large number of basic and clinical trials. But as a kind of local treatment, the recurrence rate of photodynamic is higher. Cisplatin is the most used chemotherapy drugs in clinical with easy combination with other drug, less cross resistance and low price, etc. Combination with other drugs or treatments has the sensitization effect. At home and abroad, some experiments have established that cisplatin combination with photodynamic therapy has sensitization effect. But the application in the treatment of liver cancer has not been widely recognized. This experiment was designed to investigate the treatment and sensitizing effect of cisplatin combined with photodynamic treatment on H22 liver cancer in mice, and to explore its mechanism preliminary.Method:1 H22 hepatoma cells in mice models is created and are grouped according to the method of random numbers: divided into control group(control), cisplatin group(DDP), Photodynamic group(PDT) and combination therapy(DDP+PDT) 4 groups. Each group has 10 only.2 After the start of treatment, the general indicators of tumor-burdened mice is observed. Including the life state of mice, the tumor morphology and volume, the adverse reaction in mice.3 After the start of treatment, the treatment indicators of tumor-burdened mice is observed. Every other day the tumor volume of tumor-burdened mice, tumor growth inhibition rate and the growth curve be drawed.4 After the first 14 days, four mice were executed, to tripping tumor tissue and spleen in mice, tumor weight, the tumor is weighed tumor inhibition rate and the spleen index is calculated. HE staining on tumor tissue to observe the pathological morphology. Using Flow Cytometry(FCM) detected the apoptosis and distribution rate of tumor cell in mice.5 Each group mice which were not executed conventional breeding, observed the survival time, computed the life extension rate and the survival analysis to all tumor-burdened mice.6 Statistical analyze of data: The experimental data was analysised using SPSS 17.0 statistical software. x ± s expressed as measurement data. ANOVA was used to compare among groups of samples. The data between the inspection group was analysis with S-N-K inspection. The survival analysis used the method of Kaplan-Meier. Each tests were based on P<0.05 considered statistically significant.Results:1 After treatment general index of tumor-burdened mice: The conditions of life of mice was good. The xenograft tumor growth was better than other treatment group, the adverse reaction was mild.2 The change of the tumor volume and the tumor growth inhibition rate: Before treatment, the average tumor volume(852.6656±70.91993)mm3, with no statistically significant difference(F=0.025, P=0.994). the first 14 days, each group tumor volume was obvious difference, with statistically significant difference(F=84.311, P=0.000). At the 14 th day after treatment, the tumor growth inhibition rate of control group, cisplatin group, photodynamic group and combination therapy was 26.73%, 33.75%, 52.48%.3 In each group tumor weight and the tumor inhibition rate: At the 14 th day after treatment, the tumor weight of control group, cisplatin group, Photodynamic group and combination therapy group was(6.1800±0.2258)g,(4.8450±0.3685)g,(4.1650±0.1609)g,(3.4275±0.3136)g, with no statistically significant difference(F=70.457, P=0.000). Each group the tumor inhibition rate was respectively 0, 21.60%, 32.60%, 44.53%.4 Each groups the detection of mice spleen index: The thymus index decreased in treatment group compared with the control group, But the difference of combination therapy group and photodynamic group has no statistically significant.5 Pathological observation: The number and range of necrosis of tumor cells was the most in combination therapy group. Other treatment group compared with control group, the number of cancer cells was to reduce and the cell structure of local red dye was to increase.6 Cell apoptosis and cell cycle distribution: Compared with control group, the apoptosis rate of treatment group were increased, the difference was statistically significant(P=0.000), combined treatment group was highest peak. G2/M cells was increased in cisplatin group. Photodynamic group and combined group compared with the combined group, G0/G1 phase cells was increased, and the combined group is the most obvious.7 Survival of tumor-bearing mice: Each of the treatment groups could prolong the survival time of mice, and the difference was statistically significant(F=74.856, P=0.000). The life span was the longest and the rate of life extension was the highest in the combined treatment group.Conclusion:1 Compared with other treatment group, the combined treatment group can not only inhibit tumor growth, but also reduce poisonous side reaction, high life quality and prolong the survival time.2 The combination group compared with photodynamic group, difference was statistically significant, and the side effects of tumor-burdened mice’s immunity was not further reduce.3 Cisplatin combined and photodynamic therapy effects on different cell cycle and played a synergistic role. |
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