Experimental Study Of ～(125)I Seeds Implantation In The Treatment Of Liver Cancer

As a kind of new technology, ¹²⁵I seeds implantation has gained a rapid development since 1999 in China. It has been used in comprehensive treatment of different kinds of carcinoma besides primary liver cancer, and got satisfied therapeutic effect. However, because the mechanism of low dose rate and continuous irradiation with ¹²⁵I seeds is still not clear, the advanced clinical application of ¹²⁵I seeds implantation in treatment of carcinoma is limited. The purpose of this thesis is to provide experimental basis for clinical application of ¹²⁵I seeds implantation by investigating the effect of low dose rate and continuous irradiation with ¹²⁵I seeds in inhibiting proliferation, regulating cell cycle distribution, inducing apoptosis in BEL-7402 cell line and the treatment effect, influence on proliferation, angiogenesis, apoptosis related protein of ¹²⁵I seeds brathytherapy to hepatocellular carcinoma in nude mice. The therapeutic effect of ¹²⁵I seeds brachytherapy combined with 5-FUchemotherapy seed implantation on H₂₂ xenograft in mice and effect of previous ¹²⁵Iseed implantation on H₂₂ cells xenograft transplanted in BALB/C mice are also evaluated. The thesis includes four parts as follow:PART 1: In vitro study of low dose rate and continuous irradiation with ¹²⁵I seeds to BEL-7402 cells lineObjectiveTo evaluate the effect of low dose rate and continuous irradiation with ¹²⁵I seeds...