Inhibitory Effect Of Sodium Houttuyfonate On Synovial Proliferation With Rheumatoid Arthritis

Objective To investigate the inhibitory effect of sodium houttuyfonate on synovial proliferation with rheumatoid arthritis and explore the potential mechanism of its function.Methods1.Experiment in vitro: Cultivate primary cells with RA. Divide the primary cells into five groups equally as follows: the control group, cells treated with 25μg·ml-1 SH group, cells treated with 50μg·ml-1 SH group, cells treated with 100μg·ml-1 SH group and cells treated with 200μg·ml-1SH group. SH treated groups were administered with corresponding amounts of SH as stated above, while the control group was administered with an equivalent amount of normal saline(NS), NS and SH were administered daily for 7 days in sequential. Following the final administration, the five groups of synovial cells were measured by using an MTT assay for analysis of the inhibitory rate of SH on synovial proliferation.2.Experiment in vivo: 50 wistar rats were randomly divided into five groups, they are the control group(group1), model group(group2), 130 mg · kg-1 SH-treated group(group3), 260 mg · kg-1 SH-treated group(group4), 520 mg · kg-1 SH-treated group(group5). TypeⅡcollagen and complete Freund’s adjuvant(CFA) were injected into rats to create CIA model. Arthritis index(AI) and weight were used to evaluate the inflammatory reaction in rats. The levels of IL-6, TNF-α, VEGF were measured by enyme-linked immunosorbent assay(Elisa). The synovicytes apoptosis was detected by Td T-mediated d UTP Nick-End Labeling(Tunel).Results1.Experiment in vitro: Compared with the control group, the proliferation rate ofsynovial cells were reduced markedly in the SH treated groups. And it shows dose dependent phenomenon in SH treated groups. In vitro experiment, when the concentration of SH comes to 200μg·ml-1, the inhibitory rate reaches the maximum.2.Experiment in vivo: Compared with CIA model group, AI and cytokines’ levels were decreased obviously. The apoptotic index of synovicytes was markedly increased.And it shows dose dependent phenomenon in SH treated groups. In vivo experiment,when the concentration of SH comes to 520mg·kg-1, the inhibitory rate reaches the maximum.Conclusions SH can effectively inhibit synovicytes’ proliferation in vitro, and it tends to be dose dependent. SH can stimulate rats’ synovicytes’ apoptosis, reduce arthritis index and the levels of cytokines in CIA rats.