Effects Of Resveratrol On Fat Metabolism In Mice

With the development of human lifestyle and diet structure, the proportion of high-fat foods become larger. Thus problems such as cardiovascular disease and obesity become more and more outstanding. It was reported that resveratrol(RES) has varieties of therapeutic functions such as protecting cardiovascular system, prevention and control disease of heart head bloodvessel and so on. However the mechanism of RES to regulate lipid metabolism is not fully clear. Given the vital important role of lipid metabolism in life activities of human and animals, the aim of the study is to value the function of RES in the regulation of lipid metabolism and provide some theory basis for the in-depth study of the subsequent biological functions of RES. In the present study, firstly 32 normal male Kunming mice [fed with a normal diet(ND)] were divided into four groups: the control [saline, 1 day treatment(D) and 12 days treatment(C)] and RES-injected [1 day treatment(D+R) and 12 days treatment(C+R)] which subjected to a single injection of saline or RES(30 mg/Kg body weight), respectively. At 24 hours(single injection) and 12 days after RES injection, blood samples were collected and then the body, liver and epididymal fat(EF) weights were recorded to calculate liver and EF index(LI and EFI). Secondly, 32 normal male Kunming mice were divided into four groups [normal diet(P), P+R(RES), high-fat diet(G) and G+R], treated for four weeks, then blood samples were taken, the body, liver and epididymal fat(EF) weights were recorded to calculate liver and EF index(LI and EFI) and liver samples were cryopreserved to measure the mRNA expressions of acetyl-Co A-carboxylase(ACC), fatty acid synthase(FAS) and stearoyl-Co A desaturase 1(SCD1) and fixed in 10% neutral buffered formalin to do examinations of histopathology. The results showed that single and 12 days RES treatment(30 mg/kg.bw) did not affect plasma clinical biochemistry variables(p>0.05) and the production performance of mice. However some changes were found between the groups which had been treated with RES or not. The levels of GLU and TG of the 1-day treated mice in the RES group were increased(p>0.05), and the LI of the D+R group was significantly higher than that of the D group(p<0.05), but no difference of EFI was found. The levels of TC, TG, LDL-C and HDL-C of the 12-day treated mice in the RES group were not affected significantly(p>0.05). Such results indicated that the supplementation of RES into the diet of mice fed with ND has no effect on their plasma lipids and glucose metabolites. Total concentration of plasma cholesterol increased in HFD group. Four weeks RES treatment reduced total feed intake(p<0.05). RES reduced the increased EFI in mice fed HFD(p>0.01). The mRNA level of ACC, SCD1 and FAS did not change by RES(p > 0.05). The supplementation of RES into the diet of mice fed with high-fat diet could significantly decrease the level of fatty degeneration in liver. In conclusion, the supplementation of RES into the diet of mice fed with ND has no therapeutic effect on their plasma lipids and glucose metabolites, however, in mice which are not genetically prone to development of obesity and fed with a highfat diet, the mice got a higher body weight gain, and the level of fatty degeneration in liver was decreased with RES.