Componential Analysis Of Multigrain Cordyceps Militaris Hyphae Symbionts Under Different Processing Conditions

ObjectiveTo provide a theoretical basis for the utilization of multigrain cordyceps militaris hyphae symbionts based on the analysis of active ingredients of multigrain cordyceps militaris hyphae symbionts.MethodsMultigrain cordyceps militaris mycelium was cultured by multigrain with single factor experiment method, and then it was cultured under different processing conditions, inoculation quantity, lighting duration. The active ingredients of multigrain cordyceps militaris hyphae symbionts samples were analyzed by high performance liquid chromatography, spectrophotometric method, and Kjeldahl nitrogen method. SPSS17.0 software was applied for statistical analysis.Results1. The contents of cordycepin, adenosine, cordycepic polysaccharide, and protein in multigrain cordyceps militaris hyphae symbionts(MCMHS) were significantly different(P<0.05) in diverse inoculation quantities. With the increase of the inoculation quantity, the contents of cordycepin, adenosine, and protein in MCMHS were increased first and then decreased, and their maximum contents were 3.128 mg/g, 0.336 mg/g and 170.256 mg/g respectively under the condition of 5 m L of inoculation. The maximum content of cordycepic polysaccharide was 43.445 mg/g when inoculatied with 7 m L. 2. The contents of cordycepin, adenosine, cordycepic polysaccharide, and protein in MCMHS were significantly different(P<0.001) in varied ratios of solid-liquid media. With the increase of the solid-liquid ratio, the contents of cordycepin and adenosine in MCMHS were increased first and then decreased, and the maximum contents were 3.141 mg/g and 0.336 mg/g respectively when the solid-liquid ratio was 1:2. The contents of cordycepin and adenosine were significantly different when the solid-liquid ratios were changed(P<0.05). The quantities of protein and cordycepic polysaccharide in MCMHS were increased along with the raise of solid-liquid ratio, the maximum content reached to 184.388 mg/g and 72.953 mg/g respectively when the solid-liquid ratio was 1:2.3, their content differences were statistically significant when the solid-liquid ratio changed(P<0.05).3. The contents of cordycepin, adenosine, cordycepic polysaccharide, and protein in MCMHS were significantly different(P<0.01, P<0.05) at varied ratios of wheat to cargo rice in medium. The contents of cordycepin and adenosine in MCMHS reached to the maximum values at 3.137 mg/g and 0.336 mg/g respectively, when the ratio was 1:1. The contents of cordycepic polysaccharide and protein in MCMHS reached to the maximum values at 43.771 mg/g and 182.529 mg/g respectively, when the ratio of wheat to cargo rice was 1:2. 4. The different ratios of milk powder to the media showed the significantly different contents of cordycepin, adenosine, cordycepic polysaccharide, and protein in MCMHS(P<0.001). With the increase of added milk powder, the contents of cordycepin, adenosine and protein in MCMHS were increased first and then decreased. The sharp increases of cordycepin and adenosine were found at the 1:30-1:10 ratio of milk powder to media(P<0.05). The contents of cordycepin and protein reached the maximum levels at 3.372 mg/g and 188.429 mg/g respectively when the ratio was 1:30, while the content of adenosine reached the maximum level at 0.339 mg/g when the ratio was 1:10. The contents of adenosine and protein at different growth stages were significantly different(P<0.05) compared with other proportions of added milk powder. 5. The contents of cordycepin, adenosine, cordycepic polysaccharide, and protein in MCMHS were significantly different(P<0.001) under varied lighting duration. With the increase of lighting, the contents of cordycepin and adenosine in MCMHS were increased first and then decreased and the contents were different at different growth stages(P<0.05). Lighting for 19-27 days, the contents of cordycepin and adenosine were increased for 5-6 times and reached to the maximum levels at 28.955 mg/g and 1.345 mg/g respectively. The content of protein and cordycepic polysaccharide also reached to the maximum levels at 68.02 mg/g and 194.654 mg/g respectively on 27 th day of lighting.ConclusionsThe optimal conditions for the highest contents of active components in MCMHS are as following:1. The inoculation quantity of 5 mL for the content of cordycepin, adenosine, and protein and of 7 m L for cordycepic polysaccharide. 2. The ratio of solid-liquid media for the content of cordycepin and adenosine is 1:2 and for cordycepic polysaccharide and protein is 1:2.3. 3. The ratio of wheat to cargo rice is 1:1 for cordycepin and adenosine, is 1:2 for cordycepic polysaccharide and protein. 4. Proportion of added milk power is 1:10 for cordycepin and adenosine, 1:4.3 for cordycepic polysaccharide, and 1:30 for protein.5. The optimal lighting duration is 19 to 27 days for the content of cordycepin, adenosine, cordycepic polysaccharide and protein.