Peripheral Blood Mononuclear Cell Transplantation Effects On Edema Of Cerebral Hemorrhage In Rats

Intracerebral hemorrhage(ICH) is a severe stroke subtype, with a high morbidity, mortality, morbidity and recurrence rate. There is no effective treatment so far, Cerebral edema of the surrounding tissues is the most important pathophysiological change after hemorrhage. The intracranial pressure increases. The local blood flow decreases, and the fibrinolytic function is interrupted. The cerebral edema level is closely related to the prognosis of the cerebral hemorrhage. Mononuclear cells, which contain a variety type of stem cells, ha been widely used in the cerebral ischemic stroke. Researches proved that mononuclear transplantation could attenuate brain edema after cerebral ischemic stroke. However, its effect on the cerebral hemorrhage is still not clear. There are two sources to get mononuclear cells. It could be isolated from bone marrow and peripheral blood. Bone marrow mononuclear cells are more broadly used. However, peripheral blood mononuclear cells are less technically challenging. In this study, rat cerebral hemorrhage model was established by collagenase injecting. And peripheral blood mononuclear cells were injected through tail vein injection to detect the effect on animal behavior and cerebral edema after hemorrhage. Objective:To explore the effect of peripheral blood mononuclear cell transplantation on the cerebral edema after cerebral influence of hemorrhageic stroke in rats Methods: 1.Extract PBMCs by centrifugation of density gradient, and cultivate in 1640 medium. 2. Construct the rat ICH model by injecting type Ⅶ collagenase using stereo ta Xic instrument, and screen rat model in according to m NSS score. 3. 30 model rats were divided into the PBS group and experimental group, each group including 10 rats, and take another 10 rats into untreated group.three rats of each group were killed on 0, 1, 3, 7 and 14 days after cell transplantation, of which brain tissues were detected water content. 4.24 model rats were divided into PBS and mononuclear cell groups, each group including 12 rats, and take another 12 rats into untreated group. On 1, 3, 7 and 14 days after transplantation neural function defect scale were performed. 2.On 3 days after transplantation rats in the experimental group and control group were detected inflammation of hematoma brain tissue by HE dyeing.Results 1. Compared with the control group, neural functional recovery were promoted in experimental group, of which m NSS score was lower from day 3 to 14 days(P < 0.05); 2. Compared with the control group, the brain water content of hemorrhage hemispherein experimental group was lower(P < 0.05), 3. Compared with control group, inflammatory cells infiltration around hematomain experimental group rats is lighter. Conclusion: Intravenous injection of peripheral blood mononuclear cells can reduce hemorrhagic cerebral edema and promote neural functional recovery of cerebral hemorrhage in rats.