MiR- 10b Expression And Clinical Significance In Pancreatic Cancer

Background:Pancreatic cancer ranks fourth in cancer mortality in men and women American. It is mainly in several tumor types are poor prognosis, 5 years survival rate of about 5%. Smoking, heavy alcohol consumption and diabetes, increased body mass index have been shown to increase the risk of pancreatic cancer. Pancreatic cancer family history and genetic factors also play an important role in pancreatic cancer patients reported about 10% with a family history of pancreatic cancer, although most of the genetic basis of pancreatic cancer remains unknown, but several important pancreatic cancer genes have been identified, these high penetrance genes including Brca2 gene and PALB2 gene. Pancreatic cancer is considered to be a complex and multiple genes involved in the disease, so far, has been confirmed that the abnormal expression of multiple signal transduction pathways and growth factors, there are many oncogenes and tumor suppressor genes or activated in pancreatic carcinogenesis and development play an important role in the process of. Therefore, the pathogenesis of pancreatic cancer to further explore, looking for diagnostic marker and new therapeutic targets for diagnosis and treatment of pancreatic cancer is becoming more and more important.Mirco RNAs(mi RNAs) is a highly conserved, endogenous non coding single stranded small RNA, about 19 ~ 22 bases. Its forming process has two steps: 1 through the transcription of RNA polymerase II is about hundreds to thousands of base pairs of primary mi RNA(pri-mi RNA), then pri-mi RNA is Drosha enzyme RNA endonuclease family cut growth about 65 BP hairpin precursor mi RNA(pre-mi RNA). Single stranded RNA in Dicer 2 enzyme forms under the action of two approximately 22 bases, one of the free energy of single stranded RNA and low Argonaute 2 protein(AGO2) combined with the formation of mature mi RNA. Small molecule mi RNA and target m RNA3 ’end untranslated regions of mature complete or incomplete according to play control in different ways, with high complementary target m RNA degradation, low complementary combination could inhibit the function of m RNA. Previous studies have proved that in malignant glioma, mi R- 10 B by stem cell cycle, apoptosis suppressor gene, can inhibit the proliferation and inhibit the apoptosis of malignant glioma cells. In addition, the experiment and research of tumor of liver cancer stem cells of mi R- 10 b, suggesting that mi R- 10 b increase may be involved in the occurrence and development of hepatocellular carcinoma.However, whether mi R-10 b is involved in invasion and metastasis of pancreatic cancer? Mi R-10 b play a role in tumor suppression and tumor promotion and participation mechanism of concrete is not clear in the occurrence and development of pancreatic cancer. Objective:To detect the expression of mi R-10 b in pancreatic cancer tissue and norm al pancreatic tissue, and explore their relationship with the clinicopathological f eatures of pancreatic cancer and the occurrence and development of pancreatic cancer. Methods:Collect the first affiliated hospital of zhengzhou university from Jan. to Dec. 2014 58 cases(confirmed by pathologic results 36 cases has occurred during the transfer, transfer of 22 cases) pancreatic cancer and 10 cases of normal pancreas tissue specimen, using real-time fluorescent quantitative PCR(q RT- PCR), the method of pancreatic cancer tissue and normal pancreatic tissue was detected in mi R-10 b expression situation, again to shift in pancreatic cancer group and transfer cases.According to the collected samples of clinical case characteristics(gender, age, smoking status, tumor site, tumor size, tumor differentiation degree, AJCC staging and lymph node metastasis) group, comparing mi R- 10 b expression in pancreatic cancer tissue and its basic clinical pathological characteristics of correlation, analysis of mi R- 10 b in pancreatic cancer occurs in the development of meaning. Results:1. Mi R- 10 b expression in pancreatic cancer tissue: compared with normal pancreatic tissue, transfer and transfer group of pancreatic tissue in mi R- 10 b expression level have a certain degree of increase, however, in pancreatic cancer tissue in group transfer mi R- 10 b expression level raised particularly degree(P ﹤ 0.05).2. The expression levels of mi R-10 b with pancreatic cancer clinical pathological characteristics of the analysis results show that the mi R- 10 b expression and pancreatic cancer metastasis(P﹤0.01), significantly related AJCC staging(P = 0.016), but with age, gender, smoking status, tumor size, location, and not significantly associated with the degree of differentiation; Conclusion:1. Mi R- 10 b expressed in pancreatic cancer tissue was higher than normal pancreatic tissue, especially in metastatic pancreatic cancer tissues increase obviously, suggesting mi R- 10 b in the process of the occurrence and development of pancreatic cancer may have the effect of promoting cancer gene.2. High expression of mi R- 10 b is closely related to metastasis and clinical staging of pancreatic cancer, prompt mi R- 10 b may participate in the process of pancreatic cancer metastasis.