| Sesame seed meal has abundant nutrients and comprehensive combination of amino acids. It can produce a large number of useful small peptide after removing the anti-nutritional factor, phytic acid, by microorganism fermentation method. Small peptide is a nutritive material with special physiological activity. In thin study, we researched the prominent function of fermented sesame seed meal in the aspects of vivo antioxidation and immunity, and the effect on alleviating the oxidative damage of small intestine in high-fat environment.1 Fermented sesame meal small peptides in vivo antioxidant and immune activity.56 healthy female Kunming mice (4 weeks age) were randomly divided into 7 groups,8 mice per group. Mice in normal control were given saline, and Mice in test groups were given 3 peptide and 4 peptide at different doses (2 mg,4 mg, and 8 mg)from the fermentation sesame meal purified by Sephadex G-15. The total volume for lavaging is 0.5 mL/d. Lavage continuously for 28 days and on the day of last treatment, mice were sacrificed to obtain serum and livers. The organ coefficient of liver, spleen and thymus, the activity of malondialdehyde(MDA) in serum and liver, superoxide dismutase(SOD) and glutathione peroxidase (GSH-Px) in liver were determinde. T-lymphocytes CD3T, CD4+T, CDsT volume in spleen were determined by flow cytometer. The results showed that, in mice fed sesame peptides, the organ coefficients are not changed significantly (P>0.05), MDA content in serum and liver are reduced significantly (P0.05). SOD and GSH-Px activity in liver are increased significantly. T-lymphocytes CD3+T, CD4+T cell’s numbers are not changed significantly (P> 0.05), but CD4+/CD8+ ratio was significantly increased (P <0.05) in the 4 peptides high dose group. Experimental results showed that 3 peptide and 4 peptide from fermented sesame meal all have antioxidant capacity in vivo, and 4 peptide can enhance the body’s immune activity at high level under the experiments conditions.2 Fermented sesame small peptides affect small intestine in mice antioxidant activity.112 healthy female Kunming mice (4 weeks age) were randomly divided into 14 groups,8 mice per group. Mice in base control and high-fat control were given saline, and Mice in test groups were given crude extraction,3 peptide,4 peptide and 6 peptide from the fermentation sesame meal. Lavage continuously for 28 days and on the day of last treatment, mice were sacrificed to obtain duodenal, jejunum and ileum. the activity of malondialdehyde(MDA), catalase(CAT),superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were determinde. The results showed that, in mice only fed high-fat feed, MDA content in all parts of the small intestine in mice are increased significantly (P<0.05), CAT, SOD, GSH-Px activities are reduced significantly.In mice fed sesame peptides, MDA content in duodenal are reduced significantly Comparing to that of high-fat control(P<0.05).CAT SOD and GSH-Px activity in duodenal are increased significantly Comparing to that of high-fat control Comparing to that of high-fat control, but not yet back to the level of base control. MDA content in jejunum and ileum are reduced significantly Comparing to that of high-fat control(P<0.05).CAT SOD and GSH-Px activity in jejunum and ileum are increased significantly Comparing with that of high-fat control and back to the level of base control(P>0.05)..Experimental results showed that small peptides from fermented sesame meal can improve antioxidant capacity in small intestine of mice feeding high-fat... |
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