Screening Of Bioactive Strains From Marine Microorganisms And Study The Secondary Metabolitesthe From Strain Of 01299

Objective: To study the secondary metabolites of Actinomycetes from the south China sea’s deep-sea sediments, 26 actinomycetes were researched and 13 bioactive natural compounds were found and acquired from the Pseudonocardia sp. SCSIO 01299. Accordingly better theoretical basis were available to fully develop and use of marine-derived resources.Methods: ① The randomly selected 26 newly discovered actinomycetes fermentation materials were extracted, Thin Layer Chromatography(TLC)and High Performance Liquid Chromatography(HPLC) to determine which strains with rich secondary metabolites; with antibacterial test to selected activity strains. ② strains with activity and can produce rich secondary metabolites would fermentate with large-scale, the fermentation was extracted spin dry, turn to the atmospheric column chromatography(normal phase silica gel, silica gel RP Rp-18), the suppression preparative chromatography(MPLC) and preparative high performance liquid chromatography(HPLC) separation and purification.③ The pure compounds isolated in MS, NMR spectroscopy and other modern methods to identify its structure. ④Antibacterial activity,1,1-diphenyl-picrylhydrazyl(DPPH·) radical scavenging activity and angiotensin converting enzyme inhibitory activity were tested. Results:Actinomycetes Pseudonocardia sp. SCSIO 01299 both produce rich secondary metabolites and distinct activities, which was identified as the target strains to isolated and purified and identified the secondary metabolites and obtained 13 compounds. Which contained five cyclic dipeptides: cyclo-(L-Pro-L-Tyr) dipeptide(1), cyclo-(L-Pro-L-Vol)dipeptide(2), cyclo-(L-Pro-L-Phe) dipeptide(3), cyclo-(L-Vol-L-Phe)dipeptide(4), cyclo-(L-Pro-L-Leu) dipeptide(5); a nitrous anthraquinones:deoxynyboquinone(6); an ester: phthalic anhydride diglycol ester(7);three acids: tetradecane acid(myristic acid)(8),4-hydroxy-3-methoxybenzoic acid(vanillic acid)(9),3,5-dimethoxy-4-hydroxybenzoic acid(syringic acid)(10); an alcohol:spit leaf alcohol(11) and two benzoxazolin-ketones:6-hydroxy-2-benzoxazolinone(12), 2-benzoxazolinone(13). Compound activity tests 1 to 10 show that Compounds 3 and 5 showed(DPPH·)activity with their IC50 at 9.48 and 2.85 μmol·m L-1, respectively.Compound 4 inhibited the ACE with IC50 at 0.72 μmol·m L-1, and compound 6 showed strong inhibition against Staphylococcus aureus,Gambogic aureus, Bacillus subtilis, MIC at 0.025mmol·L-1,0.05mmol·L-1, 0.05mmol·L-1. Conclusion: The study of marine actinomycetes showed rich and active metabolites, especially the strain01299 showed better antibacterial activity,(DPPH·) radical scavenging activity and angiotensin converting enzyme inhibitory activity, provides scientific basis for the use of marine resources of the drug.