The Expression And Effect Of Angiopoietin-1 On Scar During The Scar Atrophying

Objective To explore the expression and effect of angiopoietin-1 on scar during the scar atrophying. It provides a new theoretical basis and measures for clinical prevention and treatment of scar.Methods The rabbit ear scar model was made in the same position of each ear ventral center skin regardless of gender. The rabbit ears were randomly divided into four groups. Group A is defined as the no intervention group, which used for observing the expression of Ang-1, the change of scar thickness, microvascular number and mature vessels number,the ratio of the number of mature vessels and the total number of microvascular, the relationship between the ratio and scar thickness during the scar atrophying. The remaining three groups are defined as the intervention group. The rabbit ear scar models were treated at a week after wound healing. 108 pfu r Ad-4-ANGPT1, 109 pfu r Ad-4-ANGPT1, 109 pfu adenovirus vector were inject in some rabbit ear scar models respectively in group B, group C and group D. The scars have been collected at week 1, 2, 4, 8, 12. The change of scar thickness, microvascular number and mature vessels number,the ratio of the number of mature vessels and the total number of microvascular in scar were investigated by hematoxylin and eosin staining, the immunohistochemistry of CD34 staining and Western-blot.Results The expression of Ang-1 of group A increased gradually, and the highest at 2 week after epithelial change, then decreased gradually, and the lowset at week 12, which was close to normal skin expression. The scar thickness have become thick gradually, and the most thick at 4 week as(3.48±0.10)(mm), then have become thin gradually, and the most thin at week 12 as(2.84±0.09)(mm). The microvascular number increased gradually, the highest at week 4 as(11.83±2.12), then decreased, at week 12 as(7.67±1.32). The number of mature vessels increased gradually and the highest at week 12 as(2.33±0.88). The ratio of the number of mature vessels and the microvascular number was same as the chang of mature microvascular, at week 12 as(30.38%). The relationship between the expression of Ang-1 and the number of mature vessels, the expression of Ang-1 and the ratio, the ratio and scar thickness were negative correlation(P<0.05).The change of scar thickness, the total number of microvascular, the number of mature vessels, and the its ratio of intervention groups for group B, group C and group D, which is same as group A. The scar thickness was the most thick at 4 week as group A(3.48±0.10)(mm),group B(3.31±0.17)(mm),group C(3.15±0.12)(mm),group D(3.49±0.12)(mm), and the most thin at week 12 as group A(2.84±0.09)(mm),group B( 1.96±0.11)( mm), group C( 1.84±0.14)( mm), group D(2.76±0.12)(mm). The microvascular number was highest at week 4 as group A(11.83±2.12),group B(18.27±3.04),group C(18.46±3.45),group D(11.93±1.84), then decreased. The number of mature vessels increased gradually and the highest at week 12 as group A(2.33±0.88),group B(4.17±1.76),group C(5.13±1.47),group D(2.40±0.72). The ratio of the number of mature vessels and the microvascular number was same as the chang of mature microvascular, at week 12 as group A(30.38%),group B(35.34%),group C(40.62%),group D(30.92%).Variance analysis explained that there were statistically difference(P < 0.05) between every two groups, in addition to group A and group D.Conclusions 1.The relationship between expression of Ang-1 and mature vessels, expression of Ang-1 and the ratio, the ratio and scar thickness are negative correlation. Ang-1 may play an important role in the process of blood vessels mature and scar atrophy during the scar atrophy.2.Ang-1 could promote the microvascular maturation and scar atrophying.