Clinic Study Of Levels Of HIF- 1α And VEGF At Day 1, 3, 7 After Acute Brain Ischemic Infarction

ObjectiveThe project was designed to analyze levels of HIF-1α and VEGF at day 1, 3, 7 after acute brain ischemic infarction and their correlation during acute phase after brain infarction and to provide theoretical guidance for treatment of acute brain infarctions. Methods1.Sixty-seven patients with acute brain ischemic infarctions were recruited for this study from Lai Gang Geriatrics Medicine Department and Neurology Department. Diagnosis of acute brain infarctions were confirmed by brain MRI or CT findings and met standards set forth by the Fourth National Cerebral Vascular Conference and graded with American National Institutes of Health Stroke Scale. Exclusion criteria are:(1)congestive heart failure,(2)COPD,(3) severe liver or renal insufficiency,(4)antoimmune disease,(5)malignancy,(6)hemorrhagic stroke,(7)age >90. Control samples were obtained from age- and sex- matched healthy volunteers presening for annual physical exam in the same hospital.2.Venous blood were obtained from 67 stroke patients on day 1, 3, and 7 after disease onset and 43 healthy volunteers and stored at-20℃ and used within 6 months for analysis with ELIDA to examine the levels of HIF-1α and VEGF and their correlation. Experimental data were presented as mean±standard deviation(x±s), statistical test was processed by SPSS13.0 software, significant level was P<0.05. Results1.we have found that HIF-1α level is significantly increased in patients with acute ischemic infarctions in comparison to normal controls at all three time points after disease onset(day 1, 3, and 7), with peak level reached at day 1 and gradual decline through day 3 and 7.2.VEGF levels also significantly increased on day 1, 3, 7 in patients with ischemic infarctions after disease onset in comparison to normal controls.3.There is a moderate correlation between levels of HIF-1αand VEGF on day 1, 3. Conclusions:1 The levels of HIF-1α is significantly increased in peripheral venous blood in patients with acute ischemic infarctions compared to normal control, suggesting of cellular response at molecular level to ischemic event.2. The levels of VEGF is significantly increased in peripheral venous blood in patients with acute ischemic infarctions compared to normal control.The significant increase of VEGF also plays an active role in secondary protection of brain tissue.3 The positive correlation between HIF-1α and VEGF suggests an important interaction between these two critical factors during acute response phase after ischemic infarctions.