The Establishment Of The Luzhou Area Of Glycosylated Hemoglobinconsistency

bjective: Basic information Luzhou investigation related projects glycated hemoglobin, glycated hemoglobin for the establishment of Luzhou consistency foundation; 2015 First Affiliated Hospital of Sichuan University of Medical Laboratory BIORAD D-10 type glycated hemoglobin analyzer and on this basis Luzhou city and county municipal medical institutions to establish glycated hemoglobin consistency of the results, achieve mutual recognition of test results; by fresh whole blood product dispersion trend survey before and after the establishment of the consistency of glycated hemoglobin: Includes standard deviation and coefficient of variation. Methods: By questionnaire way to understand the city and county and municipal Luzhou seven hospitals(with A, B, C, D, E, F, G letters on behalf of seven hospitals Name) Basic glycated hemoglobin; Sichuan Medical University First Affiliated Hospital Laboratory glycosylated hemoglobin analyzer as a reference system, as compared to the other participants in the laboratory system, provided by the First Affiliated Hospital of Medical University in Sichuan 3 parts of the assignment of fresh whole blood calibrator 20 parts to 5 parts of the sample preparation and various concentrations of fresh whole blood product survey, first participated in laboratory determination of 3 parts of fresh whole blood calibrators, 20 parts more than 5 parts of the sample preparation and various concentrations of fresh whole blood product survey, followed by the laboratories 3 parts fresh whole blood assay results calibrator and the assignment of fresh whole blood calibrators to establish linear regression equation, linear regression equation by participating laboratories Formula calibration, the last more than 20 parts of sample products and 5 different concentrations of fresh whole blood substitute product survey linear regression equation results after calibration. By comparison criteria according to the US National Glycohemoglobin Standardization Program certified testing laboratory setting Ⅱgrade requirements: 95% confidence interval error glycated hemoglobin results with the given value bias <0.75%, compared with the reference system for the system to detect the value of the absolute value of the percentage bias less than 6%; by value between whole blood samples testing laboratories to deliver consistency ratio that compared the results are comparable within the region, using fresh five EQA way whole blood samples for testing to calculate the standard deviation and coefficient of variation, assess improvements glycated hemoglobin establish consistency before and after. Results: Investigation informed Luzhou seven major hospitals above the county level. The use of glycated hemoglobin, wherein A hospital laboratory for the US Pleasant Moss saccharification analyzer, F Hospital of the use of domestic Shanghai Huizhong MQ-2000 PT analyzer, G Hospital of the Toshiba automatic biochemical analyzer, and the remaining 4 medical institutions using US BIORAD D-10 type glycated analyzer, aware of each hospital internal quality control are carried out, because the province does not contain EQA project glycosylated hemoglobin, and therefore were not surveyed seven hospitals participated, major hospitals in good condition equipment maintenance, equipment maintenance records complete; the First Affiliated Hospital of Medical Laboratory Sichuan glycated hemoglobin analyzer consistency through the Shanghai region glycosylated hemoglobin test; glycated hemoglobin Luzhou consistent results: A, B, C, D, E, F, G seven hospitals and Sichuan Medical First Affiliated Hospital of Medical Laboratory Bryant linear regression equation was: Y=1.0254X-0.2697,Y=1.0336X-0.3726,Y=0.9941X-0.0946,Y=0.9965X-0.1297,Y=1.0178X+0.0109,Y=0.9825X+0.1615,Y=0.9792X+0.2076;coefficient:R2=0.9958,R2=0.9959,R2=0.993,R2=0.9986,R2=0.9975,R2=0.9967,R2=0.9953;95% confidence interval error is less than 0.75%, respectively, as follows:(-0.28%,0.44),(-0.25%,0.49),(-0.28%,0.56),(-0.03%,0.34),(-0.41%,0.12),(-0.32%,0.26),(-0.42%,0.32). Conclusion: Luzhou glycated hemoglobin initially established good agreement, within the scope of the cities and counties where the test results without distinction, to standardize glycated hemoglobin, and promote consistency of clinical diagnosis and treatment of diabetes.