| Aiming to promote vascular regeneration, avoiding restenosis and later thrombus, a single confluent endothelia layer is essential. EPCs play a key role for stents surface endothelialization in situ. However, due to the less number of EPCs, how to regulate EPCs from bone marrow to damage part and to promote EPCs proliferation attracts more attentions.In this paper, we design a novel Fucoidan and CD133-antibody/SDF-1 complex bio-coatings. Various materials characterization and biological evaluation has been employed on these coatings. Finally, COMSOL soft has been used to model the CD133-antibody capture EPCs and SDF-1 modulate EPCs behaviors. The detailed reseach contents were listed as follow:Firstly, CD133-antibody and Fucoidan were immobilized on the dopamine-deposited surface by a simple covalent reaction with EDC/NHS. QCM-D has been used to optimize the reaciton condition. Results showed the optimist condition for CD133-antibody and Fucoidan immobilization is pH5.4 and the EDC concentration is 20mM. XPS, CD133-FITC, AFM, WCA results revealed that SDF-1 and Fucoidan has been well-distributed grafted, and CD133-antibody&Fucoidan coating is very hydrophilic.Subsequently, hemo-compatibility has been conducted, Platelet adhesion results showed that the best Fucoidan concentration for anti-coagulant on CD133-antibody&Fucoidan is about 100μg/ml, APTTexperiment also ensured the same concentration, and the clotting time was delayed about 12s compared with Dopamine modified surface. QCM-D analysis showed the number of captured EPCs was equal with the grafted CD133-antibody concentration; the morphology of the captured EPCs will change with the total number of EPCs captured; the low flowing velocity will improve the CD133 capturing ability.Secondly, SDF-1&Fucoidan coating has been constructed by a simple covalent immobilization on the APTES modified surface. XPS results revealed SDF-1 and Fucoidan has been grafted successfully; N high-solution XPS showed that the percentage of N element increasingwith the SDF-1 grafted concentration increasing. Platelet adhesion results determined that the optimal of Fucoidan for anti-coagulant on SDF-1&Fucoidan coating is 100μg/ml. EPCs culture results revealed the 80ng/ml of SDF-1 modified sample surface displayed higher activities and more EPCs number,and the SDF-1&Fucoidan coating showed the best result of EPCs growing at SDF-1 concentration 80ng/ml and Fucoidan concentration 100μg/ml, so the multi-functional coatingswill benefit for endothelia layer fastlyformation.At last, COMSOL soft was utilized to model the behaviors of SDF regulate EPCs and CD133-antibody capture EPCs. Results analysis showed that with the CD133-antibody concentration increasing, the efficiency of EPCs captured will be improved obviously.Flowing velocity could influence the the SDF-1 diffusion obviously, and higher SDF-1 concentration will promote EPCs migrating. Combining with real experiment, this simulation will be more attractive and practicability.In a word, our paper described the CD133-antibody&Fucoidan and SDF-1&Fucoidan coatings in detail. The two multi-functional coatings possess excellent anti-coagulant; SDF-1&Fucoidan could used to accelerate the EPCs migration, proliferation. EPCs would be captured tothe CD133-antiboy modified surface. Meanwhile, we introducing a new method named COMSOL finite element simulation for analyzing and studying various molecules mechanism, which offer a meaningful guide and could optimize experiment parameter for real experiment. |
PDF Full Text Request