Clinical Study Of The Intestinal Mucosal Damage Of The Abdominal Type Of Henoch-Schonlein Purpura In Children

Objective: By measuring the levels of serum diamine oxidase and dynamic observation of abdominal pain and bloody stool time know intestinal mucosal damage and glutamine on intestinal mucosal damage of protection of the abdominal type of Henoch-Schonlein Purpura. Discuss the possible mechanism and protection mechanism, thereby, provide theoretical basis for the clinical rational measures of drug using, reduce the pain of children, shorten hospitalization time and reduce the burden of family children.Methods:1 Cases and groupsThis research was collected 90 cases diagnosed the Henoch-Schonlein Purpura and hospitalized in the pediatric kidney and immune rheumatism specialist group in the second hospital of Hebei medical university from October 2013 to December 2014. 30 cases of those were isolated skin Henoch-Schonlein Purpura and were divided into the control group, the rest of the children were the clinical manifestation of abdominal pain and bloody stool, the time of abdominal pain and bloody stool maintained within 3days. Those 60 cases were randomly divided into two groups: the routine group and the treatment group. All the research object were without cancer, crohn’s disease and other special disease, not used heparin and some chemotherapy drugs recently. Control group and routine group given anti-inflammatory, anticoagulation, allergy regular treatment, etc, did not add any intestinal mucosa protectant. The treatment group besides given conventional, added intestinal mucosa protectant(These were given orally L-Glutamine, the specification is 0.67g/bag, oral dose is as follows: children under the age of 6, half a bag of 2/day, children under the age of 9, half a bag of 3/day, children under the age of 12, a bag of 2/day, children over the age of 12, a bag of 3/day). Before and a week after treatment, all of objects were extracted 2ml fasting venous blood. During the treatment, all of objects were observed dynamically duration of abdominal pain and bloody stool duration.2 Sample collection,handling and the date measurementAll of objects were extracted 2ml fasting venous blood in the morning of 6:00 to 8:00. All venous blood were placed in the promoting coagulation tubes and then put them in the ordinary centrifugal machine, with a speed of 2500r/min after 10 min, serum samples were collected and kept them in the fridge at-80℃. Measured the level of serum diamine oxidase and recorded the duration of abdominal pain and bloody stool duration of the objects.3 Statistics analysisSPSS 17.0 was used. The levels of serum diamine oxidase of control group, routine group and treatment group accorded with normal distribution. The comparison of the levels of serum diamine oxidase was analyzed with two independent sample t test and the result was described as mean±SD. The duration of abdominal pain and bloody stool duration wered graded and the object of study of the frequency were recorded by each grade. This date was grade date and the comparison were analyzed with Mann-Whitney Test. The date was analyzed with. P value of less than 0.05 was considered as statistical significance.Results:1 Before the treatment, the comparison of serum diamine oxidase between the control group and routine group: the control group was(3914.87±67.61)pg/ml, the routine group was(5245.29±127.19)pg/ml, the result of variance analysis was t=-50.589, P=0.000, the difference between two groups was statistically significant(P<0.05).2 Before the treatment,the comparison of serum diamine oxidase between the control group and treatment group: the control group was(3914.87±67.61)pg/ml, the treatment group was(5293.7±189.74)pg/ml, the result of variance analysis was t=-37.49, P=0.000, the difference between two groups was statistically significant(P<0.05).3 The comparison of serum diamine oxidase pretherapy and post-treatment between the routine group and treatment group: the treatment group was( 700.03±166.13) pg/ml and the routine group was(535.55±133.95)pg/ml, the result of variance analysis was t=4.222, P=0.000, the difference between two groups was statistically significant(P<0.05).4 The comparison of the duration of abdominal pain between the routine group and treatment group: the result of variance analysis was Z=-2.405, P=0.016, the difference between two groups was statistically significant(P<0.05).5 The comparison of the bloody stool duration between the routine group and treatment group: the result of variance analysis was Z=-3.015, P=0.003, the difference between two groups was statistically significant(P<0.05).Conclusion:1 The rise of serum diamine oxidase in the routine group and treatment group prompts: children with the abdominal type of Henoch-Schonlein Purpura has obvious intestinal mucosal damage and levels of serum diamine oxidase can be used as a monitoring index of the intestinal mucosal damage of the abdominal type of Henoch-Schonlein Purpura.2 Children with the abdominal type of Henoch-Schonlein Purpura taking L-glutamine gung shimron acid sodium can obviously shorten the bleeding time and abdominal pain, and this prompts: adding the intestinal mucosal protectant is significance for treatment of the abdominal type of Henoch-Schonlein Purpura.