| Objective To investigate the expression of triggering receptor expressed on myeloid cells-1(TREM-1) in the liver of rats with acute necrotizing pancreatitis(ANP)-associated liver injury. And to observe the fluctuation of serum soluble triggering receptor expressed on myeloid cells-1(sTREM-1) concentration. Finally, to explore the correlation between TREM-1 and liver injury in rats with ANP.Methods Forty-eight male Sprague-Dawley(SD) rats were randomly divided into two groups:control group(C group) and ANP group (A group). Each group was divided into 4 experimental groups according to 3rd,6th,12th, 24th hour all time points. Each experimental group has 6 SD rats. ANP model was established by retrograde injection of 5% sodium taurocholate into the biliopancreatic duct, whereas the control group just opened the abdomen to turn the pancreas gently and followed by closing the abdominal cavity. Rats were sacrificed at 3rd,6th,12th and 24th hour after treatment, to take blood from the heart and to incision the specimens of liver and pancreas. The level of serum amylase (AMY), alanine aminotransferase(ALT) and aspartate aminotransferase (AST) were tested by substrate enzymatic assay. The concentration of sTREM-1 and interleukin-1beta(IL-1β) were measured by enzyme-linked immunosorbent assay(ELISA). The pathologic changes of the pancreatic and hepatic tissues were observed and graded under microscope. The expression of TREM-1 mRNA in liver was detected by Real-time quantitative Polymerase Chain Reaction (qRT-PCR). And the expression of TREM-1 protein in liver was measured by western blot(WB).Results The pathologic scores of pancreas and liver in the A group were significantly higher than that in control group at each time point(P<0.05), but there was no difference between each time point in ANP rats; The levels of serum amylase and IL-1β in group A began to increase at 3rd hour after sodium taurocholate injection, and the peak time points were at 24th hour and 6th hour respectively, they were significantly higher than that in group C of the same time(P<0.05); Serum ALT and AST concentration began to rise at 6th hour in group A, and the peak time points were at 24th hour and 6th hour respectively, they were higher in the A group at 6th,12th and 24th hour than those in control group of the same time(P< 0.05); The level of serum sTREM-1 started to rise at 3rd hour in group A. As time went on, the sTREM-1 level rising slowly, got to peak at 24th hour, they were significantly higher than that of group C at the corresponding time(P< 0.05), as well as the dose of sTREM-1 in the A group correlated with Schmidt score(r=0.481,P<0.05); The expression of TREM-1 mRNA and protein in liver of A group began to go up at 3rd hour and reach the peak within 6h, and then went along with time, the expression appeared to decline slowly, but they were still significantly higher than that of control group at the same time point(P<0.05).Conclusions The increase level of serum sTREM-1 in group A may be correlated with the severity of ANP. And TREM-1 may play an important role in ANP with liver injury. |
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