| Objective: To investigate the changes of several cytokines associated oxidative stress in Chinese people’s armed police of forces(CAPF)soldiers who participated three-months military training in the high temperature and high humidity environment.We discussed the characteristics of several cytokines associated oxidative stress.With the purpose of exploring the correlations, the study has improved the recognition of high-intensity military training, and expected to approve the evidence about preventing and treating heat stroke, and carrying out military matters scientifically.Methods: After three-month high-intensity military training with high temperature in Beijing, 37 officers and soldiers who participated in the military training have been selected randomly as high-temperature and high-intensity group(heat stressed group). Meanwhile, 32 officers and soldiers who did not participate in the military training have been selected randomly as the control group. All of them are male, at the age of 18-25 years,21±3 years old in average, military service for 2-3 years, regular diet, non vegetarian, no neurological or psychiatric disease history, no alcohol or drug abuse history. Training intensity and environment: daily training schedule is 6:00-7:00, 8:00-11:00 A.M., and 2:00-18:00 P.M. every day. When the training started, the temperature of the ground was from 40~60°C, 50°C in average. The training mainly included parade step and square array training. Blood specimens before the training and three month after training were collected respectively. The levels of NE, IGF-1, NO, NOS, ICAM-1 and IL-6 were measuring with methods of Enzyme-linked immunosorbent assay(ELISA) and chemical colorimet. Afterwards statistical software SPSS13.0 statistical analysis were applied.Results: By comparing the heat stressed group and the control group, the level of serum NE, IGF-1, NO, NOS, ICAM-1 and IL-6 in the officerses and soldiers of heat stressed group was significantly higher than in the control group. And there is a significant statistical difference between heat stressed group and normal control group. The NE serum concentration of heat stressed group and the control group respectively was 447.5 pg/ml, 316.3nmol/ml.The NOS serum concentration of heat stressed group and the control group respectively was 6.641U/ml, 1.660U/ml.The NO serum concentration of heat stress group and the control group respectively was 7.812pg/ml, 4.366pg/ml. The ICAM-1 serum concentration of heat stressed group and the control group respectively was 43.612 umol/L, 8.942umol/L.The IL-6 serum concentration of heat stressed group and the control group respectively was17.673 pg/ml, 6.335pg/ml.The IGF-1 serum concentration of heat stress group and the control group respectively was 60.480ng/ml,480.040ng/ml.Conclusion:1 high-temperature and high-intensity military training causes a significantly rise of inflammatory cytokines, included ICAM-1 and IL-6. It is a prompt that heat stress and exercise can induce systemic inflammatory response, causing a multiple organ damaged. And it will be one of the pathogenesis about the high-temperature, high-intensity training can lead to heat stress.2 The results show the serum level of NO and NOS of whom has participated in the high-temperature,high-intensity military training have raised. That explained oxidative stress and lipid peroxidation which induced tissue ischemia and hypoxia play an important role in heat stress and the development of organ damage occurs.3 The study also found the serum level of NE and IGF-1 have raised in high-temperature, high-intensity military trainers. It means neurotrophin of the central nervous system plays an important role in the progress of organ damage which induced by high-temperature, high-intensity military training. |
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