The Effect Of Julian Shengban Granule On Primary Immune Thrombocytopenia In Mice Model

Objective: Primary immune thrombocytopenia(idiopathic thrombocytopenic purpura, ITP),is one of the most common thrombocytopenic diseases. The adrenocortical hormones and immunosuppressors are main drugs for treatment of ITP. Because immunosuppressors may induce serious adverse reactions during repeated application, more and more patients instead choose Chinese medicine. In this study, an animal model of idiopathic thrombocytopenic purpura was established to investigate the effect of Julian Shengban granule on ITP.Methods: The mouse model of ITP was made with improved immune method. Healthy female BALB/C mice were randomly divided into 6 groups: normal group, model group, prednisone group, Julian Shengban granule groups with 12 mice in each group. The guinea pig anti-mouse platelet serum(GP-APS) was given by intraperitoneal injection to BALB/C mice in experimental groups every other day to establish ITP animal model. And 0.9% NS was given by intraperitoneal injection to the mice in normal group.After GP-APS administration, prednisone 10mg/kg was given by intragastric administration to the mice in positive control group once per day, Julian Shengban granule(2.5g/kg, 5g/kg and 10g/kg) was administrated ig to the mice in Julian Shengban granule groups, and the mice in normal group and model group were given equal volume of distilled water. The platelet count was determined by full-automatic blood analyzer after modeling and one week of treatment. The bone marrow smear from femur bone was stained with Wright-stain to determine the number of megakaryocyte by microscope. The bone marrow smear from femur bone was stained with Wright-stain to determine the number of megakaryocyte by microscope. The sternal bone marrow smear was stained and the change of megakaryocyte forms were observed by microscope. The content of IFN- γ and IL-4 in serum was determined by ELISA assay.Results: 1 After molding, platelet count in model group was significantly lower than that in normal group(P<0.05). After a week of treatment, compared with model group, the platelet count in prednisone group and Julian Shengban granule groups increased obviously(P<0.05), and the platelet count in prednisone group and Julian Shengban granule large-dose group was similar to that in normal group(P>0.05).2 Compared with the normal group, the megakaryocyte count in model group increased obviously(P<0.05), which was significantly decreased by prednisone and Julian Shengban granule(P<0.05). The percentage of platelet-forming megakaryocyte in model group obviously decreased compared with the normal group(P<0.05), which was significantly increased by prednisone and Julian Shengban granule(P<0.05). The platelet-forming megakaryocyte proportion in prednisone group and Julian Shengban granule large-dose group was similar to that in normal group(P>0.05).3 Compared with the normal group, the level of IFN-γ was elevated, IL-4 decreased and IFN-γ / IL-4 ratio significantly increased in model group(P<0.05), which were partially or fully reversed by prednisone and Julian Shengban granule.Conclusions:(1) This study shows that Julian Shengban granule can increase the platelet count,(2) Julian Shengban granule can inhibit the abnormal proliferation of megakaryocyte in bone marrow and increase the number of platelet-forming megakaryocyte,(3) Julian Shengban granule can regulate the imbalance of T-cell subsets and correct the disorder of immune function.Julian Shengban granule may reduce the peripheral platelet destruction by improving imbalance of T lymphocyte subsets to prevent the activation of B cells and increase the production of platelet by restraining the abnormal proliferation of megakaryocytes in bone marrow and promote differentiation of megakaryocytes into mature cells.