| Objective: In the past 50 years, as a result of the gynecology census, the morbidity and mortality of cervical cancer have fallen significantly. However, cervical cancer is still the third most common form of cancer after the breast and colon cancer. Cervical cancer is a very important cause of death in women worldwide. Human papilloma virus(HPV) infection is one of the most important causes leading to cervical cancer, about 99% of which was related with HPV-positive. Persistent HR-HPV infection is a major risk factor of the cervical cancer. The main target of this study was by investigating the expression of MCM7 and P16ink4 a in normal cervical, cervical intraepithelial neoplasia(CIN)and cervical cancer with HPV16 infection, to analyse the relationship of MCM7 and P16ink4 a in occurrence and development of cervical carcinoma and the relevance of each other, to explore clinical significance of MCM7 and P16ink4 a in the progression from CIN to cervical tumour, furthermore looking forward to providing effective markers in screening, early diagnosis, treatment, prognosis of cervical cancer.Methods:1 This study choose diagnosed 113 patients as the research object from September 2013 to December 2014 in hebei province people’s hospital of department of gynaecology clinic and hospital. All of the patients with HPV16-positive received cervical biopsy.2 Immunohistochemical staining SP method was used respectively to examine the expression of protein of MCM7 and P16ink4 a in 21 cases of or normal cervical tissues with HPV16-positive, 23 cases of low-grade lesions(CINI) with HPV16-positive, 20 cases of moderate-grade lesions(CINII) with HPV16-positive,25 cases of height-grade lesions(CINΙΙΙ) with HPV16-positive and 24 cases of cervical squamous cell carcinoma with HPV16-positive. The expression of protein of MCM7 and P16 m K4A was compared in different cervical tissues, in order to analyze the relationship of MCM7 and P16ink4 a with age, clinical stage and the cervical cancer, To explore the role of MCM7 and P16ink4 a in the progression from CIN to cervical tumour, Furthermore to provide effective markers in early diagnosis, and treatment of cervical cancer.Results:1 The overall difference in the expression of protein MCM7 in the normal cervix, CINI, CINII, CINIII and cervical cancer groups is highly statistically significant. The expression of MCM7 was significantly up-regulated in carcinomas, CINI and CINII, CINIII than normal cervical tissues, there were significant difference with each other. The expression of MCM7 protein in different cervical tissue: The expression of MCM7 protein gradually increased from normal cervical tissue to cervical cancer. MCM7 positive expression rates in 21 normal cervical tissue, 23 CINI, 20 CINII, 25 CINIII and 24 cervical squamous cell carcinoma samples were 19.05%, 52.17%, 70.00%, 88.00%, 100.00%, respectively. The expression of protein of MCM7 was significantly up-regulated in carcinomas than CINI, CINII, CINIII and normal cervical tissues, the expression of MCM7 showed significant difference between cervical cancer and CINI, CINII, CINIII, normal cervical tissues(P<0.05). The expression of MCM7 showed significant difference between CINIII and CINI, normal cervical tissues(P<0.05). The expression of MCM7 showed non difference between CINIII and CINΙΙ(P=0.103). The expression of MCM7 showed significant difference between CINII and normal cervical tissues(P<0.05). The expression of MCM7 showed non difference between CINII and CINΙ(P=0.197). The expression of MCM7 showed significant difference between CINI and normal cervical tissues(P<0.05).2 The overall difference in the expression of P16ink4 a protein in the normal cervix, CINI, CINII, CINIII and cervical cancer groups is highly statistically significant. The expression of P16ink4 a protein was significantly up-regulated in carcinomas, CINI and CINII, CINIII than normal cervical tissues, there were significant difference with each other. The expression of P16ink4 a protein in different cervical tissue: The expression of P16ink4 a protein gradually increased from normal cervical tissue to cervical cancer. P16ink4 a protein positive expression rates in 21 normal cervical tissue, 23 CINI, 20 CINII, 25 CINIII and 24 cervical squamous cell carcinoma samples were 10.52%, 30.43%, 60.00%, 80.00%, 100.00% respectively. The expression of protein of P16ink4 a was significantly up-regulated in carcinomas than CINI, CINII, CINIII and normal cervical tissues, the expression of P16ink4 a protein showed significant difference between cervical cancer and CINI, CINII, CINIII, normal cervical tissues(P<0.05). The expression of P16ink4 a showed significant difference between CINI and normal cervical tissues(P<0.05). The expression of P16ink4 a showed non difference between CINI and CINΙΙ(P=0.147). The expression of P16ink4 a showed non difference between CINIΙΙ and CINΙI(P=0.182). The expression of P16ink4 a showed significant difference between CINIΙΙ and CINΙ, normal cervical tissues(P<0.05).3 The correlation analysis of the expression of MCM7 and P16ink4 a in cervical cancer tissue with HPV16 infection: In invasive cervical cancer, the expression of MCM7 and P16ink4 a showed positive correlation.(r=0.714, P<0.01).Conclusions:The expression of MCM7 and P16ink4 a increased with the progress of cervical lesion. There was a significant difference in different cervical lesion; The expression of MCM7 and P16ink4 a were in correlation with the grades of histological in cervical squamous carcinoma, and there was a positive correlation between MCM7 and P16ink4 a in tissue of cervical cancer with HPV16-positive. There was an important meaning of predicting outcome of cervical intraepithelial neoplasia and the progression and prognosis of cervical cancer by union examining MCM7 and P16ink4 a. |
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