Aurora-A/NF-κB Signaling Involves In Radioresistance Of Human Lung Adenocarcinoma SPC-A1/DTX Cells

Background and objective:The morbidity and mortality of lung cancer occupies the first place of various cancers. It can severely impair human health and has become a global public health issue [1]. According to different pathological patterns, lung cancer is divided into small cell lung cancer and non-small cell lung cancer. Meanwhile,80% of lung cancer belongs to non-small cell lung cancer and adenocarcinoma of lung is the most common pathological pattern for non-small cell lung cancer.At present, the main treatment of adenocarcinoma of lung includes operation, radiotherapy, chemotherapy, biotherapy and molecular targeting treatment, etc. However, there are no clinical symptoms for these patients at the early stage and when these symptoms appear, it is too late to have operation. As one of the main methods to treat adenocarcinoma of lung, radiotherapy is widely applied to clinical field, but it is clinically observed that many patients are not sensitive toradiotherapy and some even resist to radiotherapy which lead to the curative effect decreased greatly. Therefore, it is significant for the clinic treatment of adenocarcinoma of lung to find a potential mechanism to resist radiotherapy.At the early stage, this research group cultivates human lung adenocarcinoma multi-resistant strainSPC-Al/DTX and also finds out that this resistant strain can resist radiotherapy compared with parent strain. This research aims to further investigate the potential molecular mechanism of human lung adenocarcinoma multi-resistant strain SPC-A1/DTX to resist radiotherapy.Methods:1. Observing the morphological difference of human adenocarcinoma of lung SPC-A1 cell and docetaxelresistant strain SPC-A1/DTX cell through optical microscope.2. Measuring the chemosensitivity outside the cells through MTT.3. Verifying the appreciation ability outside the cells through clone experiment.4. Detecting the apoptosis rate of cells at early stage through FCM (Flow Cytometry).5. Quantitatively and real-timely measuring the influence onthe expression of cell mRNA by radiotherapy through RT-PCR.6. Detecting the influence on the expression of cellular protein by radiotherapy through Western Blot.Results:1. SPC-A1/DTX cell which is irregular polygonhas a larger volume than parent SPC-A1 cell. Before cell fusion, it has many long and thin pseudopodia.2. Compared with parent SPC-A1 cell, human lung adenocarcinoma cellSPC-Al/DTX can resist radiotherapy.3. Aurora-Ajoins human lung adenocarcinoma multi-resistant strainSPC-Al/DTX cell to resist radiotherapy outside the cell.4. In human lung adenocarcinoma multi-resistant strainSPC-Al/DTX cell, nuclear transcription factorNF-KB is the downstream target gene of Aurora-A.5. NF-κB joins human lung adenocarcinoma multi-resistant strainSPC-Al/DTX cell to resist radiotherapy outside the cell.6. Aurora-Ajoins human lung adenocarcinoma multi-resistant strainSPC-Al/DTX cell to resist radiotherapy inside the cell.Conclusion:1. Aurora-A/NF-κB joins human lung adenocarcinoma multi-resistant strainSPC-Al/DTX cell to resist radiotherapy.2. Through giving rise to the abnormal activation of NF-κB, Aurora-A can further activate the signal channel of NF-κB and finally, it will cause human adenocarcinoma of lung to resist radiotherapy by virtue of a series of complicated mechanisms.