Simple Treatment Study Of Myoblasts Cell On Nude Mice Endometrial Carcinoma

Research BackgroundEndometrial carcinoma is a group of neoplasms occurred in endometrial epithelial tissue, most of which come from endometrial glands. So common in gynecological cancer in recent years, the incidence rate in the world is rising rapidly, some patients with complications can not tolerate surgery or radiation therapy, chemotherapy,in order to overcome this dilemma, clinicians have been trying to find a treatment, which not only works as we hope but also enhances the quality of life. Human myoblasts gene therapy(HMGT) can repair muscle fiber degeneration and complement genes in living cells, as the most advanced method in the world’s medical research over the previous level. In the July 14, 1990 the article published in The Lancet claimed HMGT was the Dystroph that the world’s first human gene therapy for Duchenne muscular dystrophy through repairing impaired genes. According to the data, which comes from the phase II/Ⅲ clinical trials approved by the US Food and Drug Administration, showed that myoblast transplantation in the treatment of muscular dystrophy has obvious advantages in efficacy and safety. Approved by the FDA and EMA Phase II / III clinical trials in ischemic cardiomyopathy exhibited significant efficacy and safety. Research shows that myoblast involved in insulin signaling pathways and mitochondrial biogenesis, in type II Diabetes,what’s more,it can still adjust the level of gene expression. In recent years, researches confirm myoblasts have significant therapeutic effects in the treatment of stomach cancer in animal models and urinary incontinence after radical prostatectomy.Co-culture experiments showed that the proliferation and differentiation of myoblasts can induce tumor cell cycle arrest and apoptosis in prostate cancer mouse models and injection of myoblasts can inhibit the growth and metastasis of solid tumors. Research ObjectiveTo establish nude mice endometrial carcinoma animal model, to study the interaction of myoblasts and endometrial cancer cells in nude mice, Simple to investigate the treatment of myoblasts cell on nude mice endometrial carcinoma, to provide theoretical and scientific basis for targeted therapy of endometrial carcinoma.If the basis research’s results as hoped at the beginning, the further clinical studies will be performed, and will be a breakthrough progress in the treatment of endometrial carcinoma, which will be another therapeutic means to the treatment of endometrial carcinoma beyond surgery, radiotherapy, chemotherapy, the members’ quality of life in cancer patients wil greatly improved. Research MethodsTo establish nude mice endometrial carcinoma animal model,then the nude mice were randomly divided into Experiment group, Control group and Chemotherapy group, with 10 rats in each group.the Experiment group was given intratumoral injection of myoblasts cell, the Control group was given intratumoral injection of normal saline and the Chemotherapy group was given intraperitoneal injection of Cis-platinum. Measured the neoplasm long axis and short axis once two days after the injection for 4 weeks, observed the change of neoplasm and nude mice for 4 weeks. Calculated the neoplasm volume, neoplasm inhibition rate and body weight change of nude mice. The morphology change of neoplasm tissue specimens was observed by HE Staining light microscope method. The expression of PTNE of neoplasm tissue specimens was tested by immunohistochemical detection. ResultsBefore the experiment,the nude mice weight and the neoplasm volume of Control group and Chemotherapy group, Experiment group were respectively(17.16±0. 7 3) g,( 38.27±2. 5 8) mm3,( 17.02±0. 4 0) g,( 35.99±1. 3 2) mm3,( 17.05±0. 3 8) g,( 37.05±2. 9 3) mm3. After the experiment, the nude mice weight and the neoplasm volume of the three groups were respectively( 1 6. 2 3 ±0. 3 1) g,( 1 5 4. 0 2 ±1 3. 6 6) mm3,(1 7. 2 6 ±0. 5 0)g,( 5 4. 9 4 ±1 0. 8 4) mm3,(1 9. 5 0 ±0. 4 9)g,(2 1. 6 4 ±7. 5 6) mm3. The neoplasm inhibition rate of the Experiment group and the Chemotherapy group were respectively 8 5. 9 5 %, 6 4. 3 3 %. T he positive rate of PTNE in the three groups were respectively 30%, 40%, 60%. In the Control group, the typical neoplasm cells and atypia cells were observed by HE Staining light microscope method. In the experiment group, the phenomenon of the cellular pyknosis, karyorrhexis and lots of apoptosis necrosis cells was obviously observed by the light microscope. Little of necrosis tissue was observed in the Chemotherapy group. Research Conclusions1. In the Experimental group, the neoplasm volume was significantly reduced and thespeed of growth In the experimental was slower what in Chemotherapy group, suggesting that myoblasts may be an effective clinical treatment for endometrial carcinoma.2. The morphology change of neoplasm tissue specimens was observed by HE Staining light microscope method,in the experimental group, lots of tumor lysis could be observed. The expression of PTNE of neoplasm tissue specimens was tested by immunohistochemical detection,and the expression of PTEN was more easily detected.suggesting that the experimental group was better than the Chemotherapy group in the clinical treatment for endometrial carcinoma.3. The influence of myoblast in the treatment of nude mice’ s endometrial carcinoma on body weight is positive, while in the inhibition of tumor growth, the impact on body weight, appetite, mental and other aspects is the lower. What’s more, the up-regulated expression of PTEN can be observed in the experimental group.4. The myoblasts cell and Cis-platinum can both inhibit the proliferation of endometrial carcinoma in nude mice, and the myoblasts cell is more effective. The therapy of myoblasts cell applied to the endometrial carcinoma is feasible.