| Background and ObjectiveGastric cancer is the most common malignant tumor in gastrointestinal tract tumors in our country with high degree of malignant metastasis and high mortality. Therefore, further study of the mechanism of gastric cancer and find a new early diagnosis and effective treatment is an important issue placed infront of the medical workers. Incidence of gastric cancer increases with age and increased peak age of onset is about 60 years. Compare to younger patients i.e(<40 years), the elderly patients(> 40 years) showed a high histologic biological invasion weak, low-grade malignant tumor clinical features.The difference between the two groups on the biological behavior of gastric cancer prompted the existence of different molecular mechanisms at their onset and developmental process. However, it is unclear what caused the biological characteristics of elderly gastric cancer with relatively low risk. Therefore, the study of different molecules regulating mechanism between the elderly and young patients will help us to understand the further developmental process of gastric cancer, which has the important theoretical significance for individualized prevention and treatment of gastric cancer.The characteristics of the common malignant tumors has high heterogeneity. Previous research shows that: in elderly patients with gastric cancer group is one of the main characteristic feature, the clinical pathological characteristics differ from young patients with gastric cancer, reflected it. The incidence of women is significantly lower, high degree of histological differentiation, invasive weak, low degree of malignant tumor biology. And elderly patients with gastric cancer shows genetic predisposition which is lower than the young patients with gastric cancer. The family history of gastric cancer is significantly seen in younger patients with gastric cancer group is low. On the molecular level, previous studies have found differences in the spectrum of genomic elderly patients with gastric cancer and gastric cancer among young patients. The mediated cell adhesion and maintaining cytoskeletal Ecadherin and β-Catein expression level and marked the DNA mismatch repair defects of microsatellite instability(MSI) shows significant differences between two groups of gastric cancer. However, previous studies have observed and stop at the phenomenon of these differences and lack of research on its internal precise molecular mechanisms.RTEF-1(Related Transcriptional Enhancer Factor-1)comes from family of transcription enhancer(Transcriptional Enhancer Factor,TEF). In 1996 Stewart successfully cloning and coding the TEF from the human cardiac cells for the first time and then the other members of this family continue to be discovered. The presently known family members include four:- TEF-1, RTEF-1, DTEF-1 and ETF. The family has a common highly conserved DNA binding domain, which can play its regulatory role binds to the gene promoter M-CAT originals. RTEF-1 transcriptional enhancer as an important part of the sub-family, previous studies were focused on its endothelial cells and angiogenesis regulation. The previous results of Che P confirmed the Mir-125a-5p could cause the elderly mice angiogenesis by down regulated. For the close relationship between angiogenesis and cancer, recently the RTEF- 1 role in tumor formation also caused the attention.We hypothesized that RTEF-1 and related regulatory factor expression in gastric cancer in young people and elder people has any difference or not. If it causes the difference between the elderly and young people with gastric cancer then shows the histological degree in biology and invasive tumor malignancy. AimDiscuss its significance of RTEF-1 and its upstream regulation of gene expression in gastric cancer in elderly and young people and if there is a difference or not. Materials and Methods1.The research object and specimen collection: Specimens collected from the patients undergoing radical gastrectomy in gastrointestinal surgery department of the First Affiliated Hospital of Zhengzhou University, from July 2014 to January 2015. Inclusion criteria: Preoperative examination, such as pathology and endoscopy suggest stomach malignancy. We use CT to stage for tumor. Patients receiving standard D2 resection, Postoperative pathology confirmed for poorly differentiated adenocarcinoma of stomach cancer, No chemotherapy, radiation therapy and integrative medicine and other anti-tumor therapy before surgery. This test upon the approval of the hospital ethics committee and with patients informed and signed the consent.2.From the analysis of elderly gastric cancer Microarray data results and the differential expression of mi RNAs in youth gastric cancer found that mi RNA high expressed in elderly gastric cancer and lower expression in young gastric cancer, RTq PCR validation.3.Respectively, using RT-q PCR, Western Blot technique to detect RTEF-1 m RNA and protein levels in elderly patients with gastric young primary gastric cancer cells and compared the expression.4. All results data are analyzed by SPSS17.0 software with P <0.05 as statistically significant difference.Results1.Mir-1271 are abundant and relatively significant change in gastric cancer in elderly people and young people.2.RTEF- 1 protein expression in elderly gastric cancer cell is less than it is in the young gastric cancer cell.3.The expression of mir-1271 in elderly gastric cancer cells elevated about 3.2 times than in the young gastric cancer cells.ConclusionThere are differences in the expression of mir-1271 and RTEF-1 in elderly and young patients with gastric cancer. This difference may be related to different histological and tumor biology of invasive malignancy between the elderly and young patients of gastric cancer. Results... |
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