The Related Mechanism Of Myocardial Tissue Injury Induced By Severe Acute Pancreatitis

Objective The mortality rate of myocardial injury complicated with acute severepancreatitis (SAP) is very high,which is a serious threat to the patients’ life. The aim of thisarticle is to demonstrate the specific mechanism in SAP-induced myocardial injury withthe help of molecular biology,thus to provide a theoretical basis for the development ofrational clinical use.Methods In this study, we use5%sodium taurocholate to produce a rat SAP model,80SD rats were randomly divided into two groups, sham group (sham) and modelgroups,each40and each group was set3h,6h,12h and24h.The changes of serum amylase(AMY), lactate dehydrogenase (LDH) and creatine kinase (CK-MB)were detected byautomatic biochemical analyzer, Observing the morphology of myocardial and pancreastissue with HE staining, The expression of myocardial tissue P-GSK-3β was detected byWestern blot and immunofluorescence assay, Determining the variation of myocardialmitochondria absorbance with mitochondrial swelling experiment, Observing the impact ofsodium taurocholate to the isolated rat hearts with perfused equipment.Results1.Ther serum enzyme test showed that compared to sham group, somewhat themodel group of amylase, lactate dehydrogenase and creatine kinase increased,moreover,12h elevated markedly(P <0.05).2.In HE staining, compared to sham group,pancreatic tissue damage of model groups appeared in different degrees, includinginterstitial congestion、 edema and inflammatory cell infiltration, what’smore,accompanied by cellulose exudating, bleeding and necrosis occurred in limbiclobular and pancreatic duct.similarly, in model group,myocardial tissue becamecongestion,edema and muscle gap broadening, most serious in12h, the muscle of whichalso became out of order.3.Compared to the sham group, in model group,myocardial tissueP-GSK-3β expression elevated and12h showed a significant increase(P <0.05).4.In mitochondria swelling experiment, myocardial mitochondria absorbance decreased inevery group, reduction of12h is the largest (P <0.05).5. P-GSK-3β expression of sodiumtaurocholate perfusion group and the control showed no significant difference.Conclusions1,Acute severe pancreatitis concurrent myocardial damage is most severe in12h.2, P-GSK-3play a role in the of myocardial damage in acute severe pancreatitis.3,P-GSK-3does not inhibit the mPTP opening in the rat acute severe pancreatitisconcurrent myocardial damage.