| Objectives By detecting the klothoprotein expression situation of the rat with sepsis AKImodel group, to study the pathogenesis of klothoprotein in sepsis acute kidney injury.Methods Selected72healthy male SD rats, weight about250g，8weeks of age wererandomly divided into blank control group （n=16）, CLP group（n=28）, highklotho protein expression （n=28）. control control intervention groupLP group weregave ordinary feed and water; in control group, only Find the cecum, gently squeezed afteropen the abdominal cavity; In contrast, the B group rats to do CLP operation. Theintervention group was a intervened intervention groupS, the rats were feed CS5g／（kg·d） a week before gave operation, then the rats give CLP operation. All of theoperated rats were Subcutaneous injected Levofloxacin hydrochloride20mg/kg15minbefore and3h after the CLP operation. After the operation, subcutaneous inject saline of5ml/kg to supplement the loss of fluid volume. Each group were blooded respectively inpostoperative6h,12h,24h by abdominal aorta in pursuance（six rats of each group ofeach time）, Each specimen will centrifugal and select serum to Cr yopreserve in-80℃.The serum wan Used to determine the levels of Cr and BUN. Take the venous blood tobacteria culture. The right kidney was paraffined after fixed paraffin in4%paraformaldehyde for about24to48hours, and was used to detect the expression andlocationin of klotho protein with immuNOhistochemical method. In each12hours after theoperation group, randomly pick up a mouse left kidney superiors about1cm3inglutaraldehyde to fix, used for electron microscopy detection line electron microscopy. Theremaining10rats in each group to observe the biological behavior changes and4days,7days survival rate.Results1The CLP group rats is depression, the activity is slow; Eating less;Arrectorobvious. open the abdomen, we can see more pale bloody ascites, have a stench.Association with edema, hyperemia.Intraperitoneal bowel tissue adhesion. part of the endcecum teen black. control group without the above changes. intervention grouppostoperative general situation is badder than CLP group. group D rats between A and Bgroup.2The Cr’ Each time point in intervention group serum Creatinine were significantlyhigher A, B and D group （P <0.05）, And extended gradually increase over time.groupD serum creatinine at each time point was significantly lower than intervention group andCLP group （P <0.05）, began to decline after the12h,24h close to A level （P>0.05）, But it is still slightly higher than that of control group; Compared with controlgroup, CLP group serum creatinine in6h,12h,24h increased significantly （P <0.05）.3The BUN: Compared with control group, CLP group urea nitrogen concentrations were significantly higher in Each time point（P <0.05）, Compared with CLP group andcontrol group,the urea nitrogen concentration of intervention group were significantlyincreased at each time point（P <0.05）, the group D blood urea nitrogen concentrationsignificantly were lower than the C and B control groupt each time（P <0.05）, but ithave little differences to control group at the6h point （P>0.05）, but it is stillsignificantly higher than the control group.4Compared with control group, CLP grouprenal pathological tissue damage at each time point score were significantly increased;group D6h,12h renal pathological tissue damage score significantly higher than that ofcontrol group; Compared with CLP group, group D all time points were lower, Comparedwith control group, CLP group kidney tissues at each time point, apoptosis ratesignificantly increased; Each time point Compared with CLP group, group D of renaltissue cell apoptosis rate was significantly lower, but still significantly higher than that ofcontrol group; Compared with control group, CLP group,intervention group of renalpathological tissue damage score each time point were increased.5Compared with controlgroup, CLP group,intervention group of kidney tissues at each time point klotho proteinexpression decreased obviously, the klotho protein of renal tissue of intervention groupexpression increased.6Blood bacteria culture, NO bacterial growth in control group, CLPgroup,intervention group were visible bacterial growth, can be detected escherichia coliand proteus, pseudomonas genus and small amounts of the positive cocci.7controlgroup,CLP group,intervention group4days survival rates were100%,30%,20%;intervention groupompared with CLP group although no statistically significant difference.control group,CLP group,intervention group,7days survival rates were100%,20%,30%, CLP group,intervention group there was statistical significance differenceComparing （P <0.05）..Conclusions1Cs mycelium powder can enhance septic acute renal injury rats kolthoprotein expression, has certain protective effect to the kidney.2oxidative stress injuryinvolved in the onset of septic acute kidney injury process.3Cs mycelium powder canchange the prognosis of septic acute renal injury in rats. |
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