| Objectives Diabetes is a kind of chronic metabolic disorderPersistent hyperglycemia cancause damages to tissue, leading to a variety of acute and chronic complications.Diabetes’bones are more prone to fracture,The slow or even fruitless healing process hasbeen a tough issue for doctors in all the subjects. In this study, based on the jaw bonefacture model of rats with Diabetes I, the healing process with the treatment of thecombination of alendronate sodium and insulin was observed. The influence ofbisphosphonates (alendronate sodium in this experiment) on diabetes’ fracture healing wasthen discussed, so as to provide clinical theory basis and animal experiment data for thedetermination of medicines to be used to accelerate the fracture healing for diabetespatients.Methods Choose96Wistar rats aging from6to8weeks and weighing300±20g, andrandomly divide them into4groups, namely the control group(group A), diabetes fracturegroup(group B), diabetes fracture+insulin group(group C), diabetes fracture+insulin+alendronate soodium group(group D), each group consisting of24rats. Measuring thenormal rat mandibular bone mineral density. After two weeks’ adaptive feed, large dose ofSTZ is injected into the rats’ enterocoelia all at once and the diabetes I animal model isthereby set up. Three days later, blood sugar concentration is measured at randomconditions. According to relevant literatures, if the concentration is higher than16.7mmol/L, the model is successfully built. After three days of normal feed, the fracturemodel of the rats’ left jaw is made under general anesthesia. After surgery, except theblank control group and diabetes fracture group, the rest of the groups are given insulin tocontrol blood sugar. One day later, gavage is done to Group D with35mg/kg/walendronate soodium, the other groups are given the same amount of saline. In the postsurgery stage, at the time of2w,4w,6w,8w,6rats taken from each group are executed,and ELISA method is used to measure the contents of serum OPG and TRACP, Themandibles are taken out to do bone mineral density (BMD) measurements and X-rayexaminations, after which they are fixed by4%paraformaldehyde for48h and decalcifiedby10%EDTA for60d. After decalcification, the tissue blocks are embedded in wax forfuther observation of HE karyotype, chemical examination of OPG immunologic tissue and examination of TRACP-5b specificity chromosome.Results1After the diabetes models are successfully built, there are typical symptoms ofdiabetes in the rats; After the fracture models are built, the soft tissue around the woundswells without infection., In the experiment, the blood sugar level of rats with diabetesremains stable, which shows that the fracture models are successfully set up. t2After thesuccess of model building, the X-rays of2w,4w,6w and8w are compared. It can beseen from the X-rays that at2w, the BMD at the fracture line in all groups is low; at4w,6w and8w, the blank control group and Group D present higher BMD and the fracture lineis vague. BMD comparison: At2w and4w, the BMD of Group D is much higher than thatof Group B and Group C. The data is statistically significant. Histomorphology observation:At2w,4w and6w, Group D, with less osteoclasts, is better than Group B in the number,width and length of trabeculars. Immunohistochemical detection: At2w,4w and6w, theexpression of TRACP-5b in the callus area of Group D is less than that of Group B andGroup C. The data is statistically significant.(P<0.05). OPG: Expressed in all groups, butat2w,4w and6w, the expression in Group D is higher than that in Group B and Group C.This is statistically significant (P<0.05). At8w, however, the data has no statisticaldifference (P>0.05).Conclusions1With one-time injection of STZ in large dose and surgery, the model ofmandible fracture in rats with diabetes I can be set up.2Group B shows delayed or evenfruitless healing.3Alendronate sodium, when applied to experimental models, canaccelerate the healing of fracture. The possible mechanism is that alendronate sodiumaccelerates the expression of OPG andrestrains the release of TRACP and the excessiveosteoclast proliferation in the fracture area, thereby promoting bone healing. |
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