Studyon Screening Effective Ingredients In Natural Medicine Against HIV-1Integrase

AIDS, also know as acquired immune deficiency syndrome. Since AIDs was firstfound by American researchers in1981, it was found that AIDS spread rapidly aroundthe world, and so far there is no effective treatment. AIDS has becoming a seriousthreat to human public healthy. AIDS was caused by the HIV which including twoclosely related types, called HIV-1and HIV-2, respectively, the vast majority ofAIDS is caused by HIV-1.The copy of HIV should go through serious of steps which incluing pass through,reverse transcription, integration, gene expression, assembly, budding and mature.HIV-1integrase plays a very important role in the viral replication, The majorityfunction of integrase is responsible for the concerted of linear viral DNA genome intohost chromosomes, then accomplishing the integration with the heip of virusnonstructural protein and cellular proteins. Integrase mediated the relationshipbetween virus genome with the host chromosomes, which makes it a appropriatetarget to look for HIV inhibitor.The main purpose of this study is isolate monomers which can combined withintegrase from KA-60. With the foundation of preparing high purified HIV-1integrase,we started to extraction of natural medicines KA-60. Then use Biacore SPR to screenseveral classes of extracts of KA-60against to the integrase. It shown that which partsof the extracts had a positive binding ability to integrase. Soon confirming the extractscan inhibit the HIV-1virus by MAGI test. Where after, using a protein bindlingcolumn to purfied the extracts and obtain the ingredient named KA-60-6and KA-60-7which against to integrase. Analyzing these ingredient by HPLC-MS, then comparedthe results with the database of molecular weight and screening the substance calledH,A,E may have a good effect on against HIV-1and then test them on cellular level.Trying to appraise their synergy ability by Macsynergy II. In the end computersimilating docking was employed to forecast and identity the affinity to integease byAutodock. Results showed that they all have a good binding ability with HIV-1integrase and compound H is best among them, the results can be the basis foroptimizing the structure for further research.