Chemical Constituents From The Leaves And Twigs Of Jatropha Podagrica

Jatropha species are known to be a rich source of various types of diterpenes.Some of those constitutes possess interesting biological activities includinganti-cancer, anti-bacterial, cytotoxic and insecticidal properties. Hence, in recent yearsinterest in this genus has experienced an inconceivable explosion.30compoundsincluding2novel diterpenoids,4new compounds and20known compounds wereisolated from the leaves and twigs of Jatropha podagrica. Some of those compoundswere tested for anti-bacterial, cytotoxic activities and anti-TMV activities.1. The air-dried and powdered leaves and twigs of J. podagrica were extractedwith MeOH under reflux. The solvent were evaporated under reduced pressure togive the crude extracts, which were suspended in H2O and then partitioned withpetroleum ether, EtOAc and n-butyl alcohol successively to give three correspondingportions. By various saparation methods including column chromatography oversilica gel, MCI silica gel, C18silica gel, and Sephadex LH-20, and HPLCrespectively,30compounds including2novel diterpenoids,4new compounds and22known compounds were isolated from the petroleum ether and EtOAc portions ofthe leaves and twigs of Jatropha podagrica. The structures of the new compoundswere established by using spectroscopic data, including MS, NMR, ECD, ΜV, IRdatas. The known compounds were mainly Lathyrane type diterpenoids, additionalincluding lignins and coumarins. The two novel compounds were tested foranti-bacterial and cytotoxic activities. Howere none of them showed inhibitoryactivity against those cells with IC50>40μM. Japodagricanone B showed weakeractivities against MRSA98#with an MIC value of25.0μg/mL. Some compounds(﹥20mg) were tested for anti-TMV activity by means of half leaf, the resultsshowed that those compounds had weaker anti-TMV activity, the average inhabitionrates were between10%-30%. 2. A review on the development of studies on chemical constituents anbiological activities of Jatropha genus in recent years.