| [Objective]Observed the changes of serum GLO I in type2diabetes retinopathy, anddiscuss its related to AGEs、sRAGE, explore the potential clinical significance of GLO intype2diabetic retinopathy.[Methods]53cases of type2diabetic patients who hospitalized in the Department ofEndocrinology ward of Fujian Medical University Union Hospital and28cases of controlpeople who went to physical examination center for health examination in2012October to2014April were enrolled. Divided into three groups: normal control group (N group)28cases, mean age (55.93±6.21) years,16males,12females; type2diabetic patients withoutretinopathy group (DM group)30cases, average age was (59.37±6.93) years old, male21cases, female9cases; type2diabetes mellitus with retinopathy group (DR group)23cases,mean age (59.39±7.41) years old, male12cases, female11cases. Collecting data of eachresearch object: weight, height, blood pressure, duration of disease, past medical history,diabetes other complications, medication history, body mass index (BMI, BMI=body weight(kg)/height2(M2)); fasting blood glucose (FBG), glycosylated hemoglobin (HbA1c),triglyceride (TG), cholesterol (CHOL), high density lipoprotein cholesterol (HDL-C), lowdensity lipoprotein cholesterol (LDL-C); synchronization using enzyme linkedimmunosorbent assay (ELISA) for determination of GLO I, AGEs, sRAGE content.Patientswho had diagnosed type2diabetes must do detailed eye examinations such as best correctedvisual acuity,binocular non contact tonometry, slit lamp eye, cornea, pupil, lens aftermydriasis, slit lamp microscope VOLK front mirror fundus examination, fundus photographyin the Department of ophthalmology.Statistical analysis was performed using SPSS19.0statistical software,Single factor variance analysis was used for continuous variables;χ2testfor categorical variables; differences and correlation among the indexes were using linearcorrelation analysis and multiple linear regression analysis.Two-sided P-values <0.05wasconsidered statistically significant. [Results]1.Analysis of clinical data and biochemical indexes by single factor varianceanalysis: age of each group (group N:55.93±6.21years, group DM:59.37±6.93years,groupDR:59.39±7.41years), triglyceride (group N:2.63±1.68mmol/l, group DM:2.24±2.18mmol/l, group DR:1.51±1.45mmol/l), low density lipoprotein cholesterol (group N:2.80±0.46mmol/, group DM:3.09±0.83mmol/l, group DR:3.23±1.33mmol/l),glycosylated hemoglobin (group DM:9.04±2.70%, group DR:9.48±1.92%) difference wasnot statistically significant (P>0.05), the duration of group DM was significantly shorter thangroup DR (group DM:7.59±6.18years,group DR:11.65±8.46years; P<0.01);BMI andsystolic blood pressure in group N were significantly lower than group DM and group DN(BMI:group:N:22.40±2.16kg/m2, group DM:25±2.88kg/m2, group DR:24.87±3.19kg/m2; systolic blood pressure: group N:119.18±15.37mmHg, group DM:135.03±19.83mmHg, group DR:144.04±23.69mmHg; both P<0.01);diastolic blood pressure ingroup N was lower than that in group DM (group N:73.68±10.05mmHg, group DM:81.10±7.06mmHg, group DR:81.39±13.13mmHg; P<0.05); cholesterol, fasting blood glucose ingroup N were significantly lower than that of group DM and group DR (cholesterol: N group:2.88±1.71mmol/l, group DM:4.81±0.97mmol/l, group DR:9.24±3.47mmol/l; fastingblood glucose: group N:5.11±0.39mmol/l, group DM:8.61±3.85mmol/l, group DR:9.24±3.47mmol/l; both P<0.01), high density lipoprotein cholesterol in group N was higher thanthat in group DM (group N:1.43±0.24mmol/l, group DM:1.12±0.27mmol/l, group DR:1.28±0.32mmol/l; P<0.01).2.The constituent ratio difference of other diabetes complications, medication historybetween group DM and group DR were analyzing by χ2test showed that: diabeticmacrovascular complications, diabetic peripheral neuropathy, diabetic nephropathy and oralhypoglycemic drugs, high blood pressure in group DM and group DN were not statisticallysignificant difference (P>0.05).3.Difference of GLO I, AGEs, LN (sRAGE) between the3groups analyzing by singlefactor variance analysis showed that: GLO I (group N:283.63±62.60ng/ml, group DM:247.59±46.19ng/ml, group DR:205.81±43.71ng/ml), AGE (group N:1119.77±427.65ng/ml, group DM:1741±354.20ng/ml, group DR:2066±599.70ng/ml), LN (sRAGE) (group N:7.12±0.69ng/l, group DM:6.36±0.37ng/l, group DR:6.13±0.27ng/l) insignificant difference between N group, DM group, DR group (P<0.01), and GLO I, LN（sRAGE）in N group, DM group, DR group are gradually decreasing trend,while AGEsbetween the three groups, gradually increasing trend.4.The correlation between AGEs, GLO I, LN (sRAGE) and the general information andbiochemical parameters using Pearson correlation analysis showed: GLO I was negativelyrelated with BMI, systolic blood pressure, cholesterol, low density lipoprotein cholesterol,fasting blood glucose,AGEs, but positively correlated with LN(sRAGE);AGEs wasnegatively related with GLO I,LN (sRAGE),but positively correlated with age,BMI,diastolicblood pressure, fasting blood glucose;LN (sRAGE) was negatively related with BMI, systolicblood pressure, diastolic blood pressure, cholesterol, fasting blood glucose, AGEs,butpositively correlated with triglyceride and GLO I.5.Multiple linear regression analysis of GLO I, AGEs, LN（sRAGE） showed: the subjectgroup, BMI, systolic blood pressure, cholesterol, low density lipoprotein cholesterol, fastingblood glucose as variables, GLO I as the dependent variable for multiple linear regressionshow that: subject groups of GLO I (P<0.01) have a significant effect, namely, according tothe N group in DM group, DR group, the packet sequence GLO I level decreased31.15ng/ml;Then subject group, age, BMI, systolic blood pressure, diastolic blood pressure, cholesterol,fasting blood glucose as variables, AGEs as dependent variables for multivariate linearregression show that: subject to group AGEs (P<0.001) have a significant effect, namelyaccording to the packet sequence AGEs level of N group, DM group, DR group graduallyincreased to417.94ng/ml; subject group,BMI, subjects, systolic blood pressure, diastolicblood pressure, triglyceride, cholesterol, fasting blood glucose as variables, LN (sRAGE) asthe dependent variable for multiple regression showed that: subject group have a significanteffect to LN (sRAGE)(P<0.01),namely. in group N, group DM, group DR packet sequenceLN (sRAGE) level decreased0.296ng/l.[Conclusion]The level of serum GLO、LN（sRAGE） in diabetic retinopathy patients wassignificantly decreased, and negative correlation with AGEs,sRAGE in serum, indicatingthat GLO I may play an important role in the inhibition of type2diabetic retinopathy occurrence and development. |
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