| [Objective] Observed the changes of serum GLO1in type2diabetes peripheral neuropathy, anddiscuss its related to oxidative stress indexes and AGEs、sRAGE, explore the potential clinicalsignificance of GLO in type2diabetic peripheral neuropathy.[Methods] Select89cases with type2diabetic who hospitalized in the Department ofEndocrinology ward of Fujian Medical University Affiliated Union Hospital from March2012toNovember2013, including31cases of type2diabetes without peripheral neuropathy (2group or DPN0group), the average age (57.39±2.94) years and58cases of type2diabetes with peripheral neuropathy(3group or DPN1group), the average age (57.38±2.09) years old, and50cases who underwent healthyexamination and showed no diabetes and its complications served as normal control group(1group orNC group), the average age (55.4±1.47) years．Each patient is measured clinical data like the height,weight, blood pressure, fasting blood glucose, glycosylated hemoglobin(HbA1c), triglyceride (TG),cholesterin(CHOL), high density lipoprotein cholesterol(HDL-C), low density lipoproteincholesterol(LDL-C), nerve conduction velocity, determined GLO.AGEs,sRAGE in serum withELISA method and measured oxidative stress indexes like MDA, SOD, GSH－PX. Using SPSS17.0statistical software for statistical analysis, measurement data using completely random design of singlefactor analysis of variance (ANOVA), count data by chi-square test, correlation analysis using linearcorrelation. Compare the differences and correlation among all the indicators in each group.[Results]1.Clinical data: Single factor variance analysis showed that age between the three groups(NC group55.4+/-1.47years old, DPN0group57.39+/-2.94years old, DPN1group57.38+/-2.09years old；P=0.290), the GHOL (NC group4.47+/-0.18mmol/l, DPN0group4.54+/-0.33mmol/l, DPN1group4.68+/-0.21mmol/l; P=0.360), LDL-C (2.81+/-0.14mmol/l for NC group,DPN0group2.61+/-0.3mmol/l, DPN1group2.87+/-0.21mmol/l; P=0.233) there was nosignificant difference; Patients with type2diabetes, BMI (DPN0group24.36+/-1.02kg/m2, DPN1group23.92+/-0.76kg/m2), systolic blood pressure (131.45+/-6.00mmHg DPN0group, DPN1group132.71+/-4.1mmHg), diastolic blood pressure (81.65+/-2.56mmHg DPN0group, DPN1group81.76+/-2.48mmHg), TG (DPN0group1.73+/-0.31mmol/l, DPN1group and1.62+/- 0.21mmol/l) is significantly higher than healthy controls (BMI22.65+/-0.55kg/m2, systolic bloodpressure of22.65+/-2.84mmHg, diastolic pressure is74.58+/-2.13mmHg, TG1.08+/-0.12mmol/l, BMI, P <0.05, more than all of P <0.01), HDL-C (DPN0group1.21+/-0.14mmol/l,DPN1group1.12+/-0.08mmol/l) Significantly lower than that of healthy controls (1.49+/-0.07mmol/l; P <0.01); DPN0group with diabetes duration (6.42+/-1.75years), FBG (7.37+/-0.55mmol/l), HbA1c (8.06+/-0.79%) than group DPN1above index increased significantly,(8.72+/-1.34years course; P <0.05, FBG10.68+/-0.78mmol/l; P <0.01, HbA1c9.36+/-0.55%; P <0.01),significantly reduce phil total nerve conduction velocity (MCV and SCV DPN0group were50.00+/-1.89, and58.85+/-2.41m/s; DPN1group were44.54+/-2.05,17.96+/-6.94m/s, P <0.01).2. Single factor analysis of variance showed a significant rise in DPN1group which serum AGEslevels (1220.82+/-102.63ng/ml NC group,DPN0group1552.63+/-147.67ng/ml, DPN1group1984.65+/-129.38ng/ml; P <0.01); While DPN1group GLO and sRAGE in serum weresignificantly reduced (respectively NC group291.13+/-19.42ng/ml, DPN0248.75+/-24.78, DPN1group212.96+/-16.3ng/ml, P <0.05; and NC group1663.23+/-142.64ng/l, DPN0686.78+/-95.96ng/l,495.61+/-55.8ng/l DPN1group, P <0.01). Pearson correlation analysis showed that theGLO,sRAGE, HDL-C, PNCV are related, and AGEs, systolic pressure, diastolic pressure, FBGnegative correlation (all P <0.05).3. Oxidative stress indicators: Single factor analysis of variance showed that diabetes group ofserum MDA level (DPN06.39+/-0.44nmol/ml, DPN1group8.44+/-0.65nmol/ml) the NC groupsignificantly increased (4.28+0.37nmol/ml); and SOD, GSH-PX (DPN0, DPN1group respectively:SOD to10.17+/-1.09,1.09+0.63U/ml, GSH-PX119.09+/-4.84,4.84+/-5.62energy units) theNC group (SOD13.07+0.9U/ml;138.19+/-9.15GSH-PX activity units) decreased significantly.Diabetes, according to the comparison between the two groups DPN1group serum MDA levelsignificantly increased the DPN0group (group DPN06.39+/-0.44nmol/ml,8.44+/-0.65nmolDPN1group/ml), and SOD, GSH-PX, significantly reduced (DPN0, DPN1group respectively: SOD to10.17+/-1.09,1.09+0.63U/ml, GSH-PX119.09+/-4.84,4.84+/-5.62energy units). Pearsoncorrelation analysis showed that: the MDA, PNCV, GLO and sRAGE negatively related, AGEs isrelated; SOD, GSH-PX and PNCV, GLO and sRAGE are related, and AGEs negative correlation。[Conclusion] Diabetic peripheral neuropathy patients serum GLO significantly reduced, and isassociated with AGEs, sRAGE level in serum and oxidative stress, prompt GLO may occur in the inhibition of diabetic peripheral neuropathy, and plays an important role in development. |
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