Study On The Anti-infection Effect Of Compound Traditional Chinese Medicine NZF

Nowadays, the incidence of clinical infection diseases caused by bacteria is showing atrend of rising year by year. With the antibiotics abusing in humans and livestock farming,drug-resistant strains of bacterial and drug residues in animal source foods have attractedmuch attention. So people in the pharmaceutical industry urgently hope to find newtraditional Chinese medicine, which have some advantages and features, such asresource-rich, lower toxicity and no easy to form resistance, etc.On the basis of preliminary studies and the theory of traditional Chinese medicine.This paper intends to filter out the significant microbial activity compound as NZF byresearching antimicrobial activity in vitro of single Chinese herb and its compounds againsthuman and aquaculture’s common pathogens.Take the bacteria-inhibiting in vitro as aguide to study the extraction and purification process of NZF. We hope to filter out theantimicrobial active substances, and provide the experimental basis of anti-infective drugdiscovery, as well as the basis of the application evidence in the aquaculture industry. Theresults of the study are as follows:（1）Study on the prescription of NZF: Bacteria-inhibiting in vitro of the singleChinese herb of NZ、HZ、BZ、SL and its compounds extracted by different solvents, weretake Staphylococcus aureus, Shigella flexneri, Klebsiella pneumoniae, Escherichia coli,Salmonella typhimurium, Salmonella enteritidis, Candida albicans,Vibrio parahaemolyticus,Vibrio alginolyiicus as the tested strains, through filter paper method and micro-dilutionmethod, we measure the inhibition zone diameter, the minimum inhibitoryconcentration(MIC), and the minimum bactericidal concentration(MBC) of antimicrobialactivity evaluation in the vitro antibacterial study, in order to screen the significantantimicrobial activity NZF prescription, as well as obtain the optimal compatibility proportion of NZF prescription.（2）Study on the optimum extraction process of NZF Prescription: To optimized theextraction method, we take the levels of activity substance W and the antibacterial activityin vitro as the investigating index. The single factor investigation (extraction method,solvent concentration, extraction times and weight solvent ratio) was used to study theeffects of extraction method. As a result, with M2method to extract1h, the level ofsubstance W is the highest, as well as the significant antimicrobial activity. Subsequently,the optimal parameters of extraction were obtained by the orthogonal experiments.Theoptimal extracting methods was confirmed: Y-solvent concentration g%, weight solventratio1:10, M2method to extract twice, one hour each time.（3）Bactericidal Effects in vitro of different fractions: Using two-phase solventextraction and purification NZF extract,the fractions obtained respectively by S-solvent,E-solvent, X-solvent and Z-solvent extraction were screened for antibacterial activity.From this source, the fraction of X showed a broad spectrum of antibacterial activity.In vitro bacteriostasis experiment results show that NZF has good antibacterial effect,especially on Staphylococcus aureus,Vibrio parahaemolyticus and Vibrio alginolyiicus,and its antibacterial effective parts mainly concentrated in fraction of X. As our researchshowed, NZF have a good antibacterial effect, and subsequent research are needed foropening new opportunities to develop pharmaceuticals based on NZF.