The Treatment Of Low Frequency Pulsed Magnetic Fields On Lower Limb Arteriosclerosis Occlusion Syndrome

Objective:To investigate the effects of low frequency pulsed magnetic field on VEGF levels in the blood and new blood vessels form, VEGF expression in ischemic tissue in the rabbit model of acute lower limb ischemia. Methods:24healthy male New Zealand white rabbit will be given high fat feed daily per120g,6weeks. Ligation the right common iliac artery to making the rabbit model of acute lower limb ischemia, Then make the rabbit model random allocation into groups as the different magnetic field strength (12mT and6mT,3mT, OmT) and magnetic field effect time (3d,5d,7d), After these steps, we phlebotomize from the vena cava and cut out muscle tissue in acute ischemia side. ELISA method to detect the contents of serum VEGF, the muscle tissue in acute ischemia side make immunohistochemical, IPP6.0testing the IOD of VEGF and the number of newborn capillaries in the ischemic muscle tissue immunohistochemical slice, use the SPSS17.0to do statistical analysis. Results:When the strength of low frequency pulse magnetic field is12mT, time is3d, the expression of VEGF in acute ischemic lower limbs muscle quantity (33933±12839) significantly higher than the other groups, statistically difference between with other groups(P<0.05), the number of newborn capillaries(76±12) is markedly increased, statistically difference between with other groups (P<0.05).At the same time, the experimental groups did not significantly increased content of VEGF in blood. Conclusions:Low frequency pulse magnetic field can promote the production of VEGF in acute ischemic limb muscle tissue and secretion, promote the acute ischemic limb local new blood vessels to form, at the same time avoided of the side effects of the increases VEGF levels in the blood.