The Effect Of Static Magnetic Field On Deep Wound Healing Of SD Rats

Objective:To investigate the effect of static magnetic field on deep wound healing of SD rats;to investigate the effect of static magnetic field on VEGF during the course of wound healing;to investigate the effect of different strength static magnetic field on deep wound healing of SD rats.Methods:Dividing forty-eight SD rats into three groups:group with 0.16T magnetic disk treated(0.16Tgroup);group with 0.32T magnetic disk treated(0.32Tgroup);control group.General wounds healing situation was observated on the third sixth ninth twelfth day ;The erea of every wound was calculated;Every granulation tissue sample was observated by tissue-pathology.The tissue of granulation was dyeing by immune tissue chemical decoration method,in which VEGF protein content with its range in tissue was measured.Results:General observation showed:There were more oozed thing and granulation in the wounds of 0.16T group and 0.32T group than in that of control group during the course of wound healing.Epithelium cell emerged more early in magnetic group than in control group. The healing time of magnetic group was shorter than of control group.Contrast 0.32T group to 0.16T group,there were more oozed thing and granulation in the wounds.The healing index of magnetic group wounds were larger than that of control group on 3rd,6th,9th and 12th day. The healing index of 0.32T group wounds were larger than that of 0.16T group wounds.Observation of histology indicated:There were more micrangium and fibroblast in magnetic group wounds than that in control group wounds during early time.In the 0.32T group were there the most micrangium and fibroblast.It was more obviously during the middle time.There were lesser micrangium and fibroblast in magnetic group on 12th day than that on 9th day.And more collagen fibers emerged. Epithelium tissue crawled obviously.It was almost closed.The amount of micrangium and fibroblast were more in magnetic group wounds than that in control group wounds.The difference has statistical significance.It was also between 0.32T group and 0.16T group.Observation of VEGF at the course of wound healing:The expressing of VEGF in magnetic group wounds on 3rd and 6th was stronger than in control group wounds.The difference between them has statistical significance.While there were no obvious difference between them on 9th and 12th day(P>0.05).The expressing of VEGF in 0.32T group on 3rd and 6th day was stronger than in 0.16T group. The difference between them has statistical significance.The expressing strength of VEGF in magnetic group reached the peak amplitude on the 6th day.That in control group reached peak amplitude on 9th day.And the peak amplitude of magnetic group was stronger than that of control group.Conclusion:1,Static magnetic disc of 0.16T and 0.32T can promote deep woundof SD rats heal.2,The mechanism of static magnetic field promoting wound heal maybe relative to the expressing highly of VEGF during early and middle time.3,The expressing of VEGF in 0.32T group wounds during early andmiddle time was stronger than that in 0.16T group wounds.lt may be because that the strong magnetic field promote directional migration of fibroblast and vascular endothelial cell quickly.